| Autor: |
Elijah Kolawole Oladipo, Olawumi Elizabeth Akindiya, Glory Jesudara Oluwasanya, Gideon Mayowa Akanbi, Seun Elijah Olufemi, Daniel Adewole Adediran, Favour Oluwadara Bamigboye, Rasidat Oyindamola Aremu, Kehinde Temitope Kolapo, Jerry Ayobami Oluwasegun, Hezekiah Oluwajoba Awobiyi, Esther Moradeyo Jimah, Boluwatife Ayobami Irewolede, Elizabeth Oluwatoyin Folakanmi, Odunola Abimbola Olubodun, Samuel Adebowale Akintibubo, Foluso Daniel Odunlami, Taiwo Ooreoluwa Ojo, Omodamola Paulina Akinro, Oluwaseun Samuel Hezikiah, Adenike Titilayo Olayinka, Grace Asegunloluwa Abiala, Akindele Felix Idowu, James Akinwunmi Ogunniran, Mary Omotoyinbo Ikuomola, Hadijat Motunrayo Adegoke, Usman Abiodun Idowu, Oluwaseyi Paul Olaniyan, Olutoyin Omolara Bamigboye, Sunday Babatunde Akinde, Musa Oladayo Babalola |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Immunogenetics. |
| ISSN: |
1432-1211 |
| Popis: |
The bacteria Vibrio cholerae causes cholera, an acute diarrheal infection that can lead to dehydration and even death. Over 100,000 people die each year as a result of epidemic diseases; vaccination has emerged as a successful strategy for combating cholera. This study uses bioinformatics tools to create a multi-epitope vaccine against cholera infection using five structural polyproteins from the V. cholerae (CTB, TCPA, TCPF, OMPU, and OMPW). The antigenic retrieved protein sequence were analyzed using BCPred and IEDB bioinformatics tools to predict B cell and T cell epitopes, respectively, which were then linked with flexible linkers together with an adjuvant to boost it immunogenicity. The construct has a theoretical PI of 6.09, a molecular weight of 53.85 kDa, and an estimated half-life for mammalian reticulocytes in vitro of 4.4 h. These results demonstrate the construct's longevity. The vaccine design was docked against the human toll-like receptor (TLR) to evaluate compatibility and effectiveness; also other additional post-vaccination assessments were carried out on the designed vaccine. Through in silico cloning, its expression was determined. The results show that it has a CAI value of 0.1 and GC contents of 58.97% which established the adequate expression and downstream processing of the vaccine construct, and our research demonstrated that the multi-epitope subunit vaccine exhibits antigenic characteristics. Additionally, we carried out an in silico immunological simulation to examine the immune reaction to an injection. Our results strongly suggest that the vaccine candidate on further validation would induce immune response against the V. cholerae infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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