Cloning and expression of rat fucosyltransferase VII at sites of inflammation
| Autor: | Risto Renkonen, Jaana Niittymäki, Pirkko Mattila |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Fucosyltransferase Lymphoid Tissue Molecular Sequence Data Oligosaccharides In situ hybridization Biology Molecular cloning Fucose Pathology and Forensic Medicine Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Chlorocebus aethiops medicine Animals Immunology and Allergy Amino Acid Sequence RNA Messenger Cloning Molecular Sialyl Lewis X Antigen 030304 developmental biology Inflammation Cloning chemistry.chemical_classification 0303 health sciences medicine.diagnostic_test General Medicine Fucosyltransferases Molecular biology Rats 3. Good health Sialyl-Lewis X Enzyme chemistry Biochemistry COS Cells biology.protein 030217 neurology & neurosurgery |
| Zdroj: | APMIS. 113:613-620 |
| ISSN: | 1600-0463 0903-4641 |
| DOI: | 10.1111/j.1600-0463.2005.apm_279.x |
| Popis: | The sialyl Lewis x (NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc) determinants serve as ligands in the selectin-mediated adhesion of leukocytes to activated endothelium. The final step in the sialyl Lewis x synthesis is catalyzed by alpha1-3-fucosyltransferase, which transfers fucose to sialylated type 2 chain. We report the cloning of rat alpha1-3-fucosyltransferase gene (rFUT) isolated from rat lymph node and kidney allograft. The rFUT is expressed as two splice variants, but only the long one showed enzymatic activity towards sialylated lactosamine. Also flow cytometry analysis with the sLex mAbs indicated that the cloned rFuc-T was a functional enzyme and a member of the Fuc-TVII family. The rFuc-TVII mRNA expression level was strongly enhanced during acute inflammatory reaction induced by kidney allograft rejection, which could be detected by in situ hybridization and quantitative real-time PCR. |
| Databáze: | OpenAIRE |
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