Genomic Maps and Comparative Analysis of Histone Modifications in Human and Mouse
| Autor: | Edward J. Kulbokas, Stuart L. Schreiber, Michael Kamal, Scott McMahon, Dana J. Huebert, Eric S. Lander, Stefan Bekiranov, Dione K. Bailey, Thomas R. Gingeras, Elinor K. Karlsson, Kerstin Lindblad-Toh, Bradley E. Bernstein |
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| Rok vydání: | 2005 |
| Předmět: |
Histone H3 Lysine 4
Chromosomes Human Pair 21 Chromosomes Human Pair 22 Methylation General Biochemistry Genetics and Molecular Biology Histones Mice 03 medical and health sciences 0302 clinical medicine Histone H2A Histone methylation Protein methylation Animals Humans Histone code 030304 developmental biology Epigenomics Homeodomain Proteins Genetics 0303 health sciences Genome biology Biochemistry Genetics and Molecular Biology(all) Lysine Chromosome Mapping Acetylation Chromatin Receptors Interleukin-4 Histone 030220 oncology & carcinogenesis Histone methyltransferase biology.protein |
| Zdroj: | Cell. 120:169-181 |
| ISSN: | 0092-8674 |
| Popis: | We mapped histone H3 lysine 4 di- and trimethylation and lysine 9/14 acetylation across the nonrepetitive portions of human chromosomes 21 and 22 and compared patterns of lysine 4 dimethylation for several orthologous human and mouse loci. Both chromosomes show punctate sites enriched for modified histones. Sites showing trimethylation correlate with transcription starts, while those showing mainly dimethylation occur elsewhere in the vicinity of active genes. Punctate methylation patterns are also evident at the cytokine and IL-4 receptor loci. The Hox clusters present a strikingly different picture, with broad lysine 4-methylated regions that overlay multiple active genes. We suggest these regions represent active chromatin domains required for the maintenance of Hox gene expression. Methylation patterns at orthologous loci are strongly conserved between human and mouse even though many methylated sites do not show sequence conservation notably higher than background. This suggests that the DNA elements that direct the methylation represent only a small fraction of the region or lie at some distance from the site. |
| Databáze: | OpenAIRE |
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