Gene expression of the liver in response to chronic hypoxia
| Autor: | Karen Schlauch, Jack P. Hayes, Monica M. Baze |
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| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty Microarray Physiology Biology Real-Time Polymerase Chain Reaction Acclimatization Internal medicine Gene expression Genetics medicine Animals RNA Messenger Hypoxia Research Articles Oligonucleotide Array Sequence Analysis Regulation of gene expression Principal Component Analysis Gene Expression Profiling Body Weight Oxygen transport Reproducibility of Results Lipid metabolism Hypoxia (medical) Hematopoietic Stem Cells Gene expression profiling Mice Inbred C57BL Endocrinology Gene Expression Regulation Hematocrit Liver Immunology Chronic Disease medicine.symptom |
| Zdroj: | Physiological genomics. 41(3) |
| ISSN: | 1531-2267 |
| Popis: | Hypoxia is an important ecological, evolutionary, and biomedical stressor. While physiological acclimatization of mammals to hypoxia is well studied, the variation in gene expression that underlies acclimatization is not well studied. We acclimatized inbred mice for 32 days to hypoxic conditions that simulated altitudes of 1400, 3000, and 4500 m. We used oligonucleotide microarrays to measure changes in steady-state abundance of mRNA in the livers of these mice. Mice exposed to more severe hypoxia (simulated altitude of 4500 m) were smaller in mass and had higher hematocrit than mice exposed to less severe hypoxia. ANOVA and false discovery rate tests indicated that 580 genes were significantly differentially expressed in response to chronic hypoxia. Few of these 580 genes have previously been reported to respond to hypoxia. In contrast, many of these 580 genes belonged to same functional groups typically respond to acute hypoxia. That is, both chronic and acute hypoxia elicit changes in transcript abundance for genes involved in angiogenesis, glycolysis, lipid metabolism, carbohydrate metabolism, and protein amino acid phosphorylation, but the particular genes affected by the two types of hypoxia were mostly different. Numerous genes affecting the immune system were differentially expressed in response to chronic hypoxia, which supports recently proposed hypotheses that link immune function and hypoxia. Furthermore, our results discovered novel elevated mRNA abundance of genes involved in hematopoiesis and oxygen transport not reported previously, but consistent with extreme hematocrits found in hypoxic mice. |
| Databáze: | OpenAIRE |
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