Increased expression of tumor necrosis factor-α converting enzyme and tumor necrosis factor-α in peripheral blood mononuclear cells in patients with advanced congestive heart failure
| Autor: | Katsuhiko Hiramori, Yudai Shimoda, Tomonari Akatsu, Mamoru Satoh, Motoyuki Nakamura, Junji Iwasaka |
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| Rok vydání: | 2004 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Enzyme-Linked Immunosorbent Assay ADAM17 Protein Severity of Illness Index Peripheral blood mononuclear cell Flow cytometry Pathogenesis chemistry.chemical_compound Internal medicine medicine Humans RNA Messenger cardiovascular diseases Aged Heart Failure Metalloproteinase medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha business.industry Middle Aged Flow Cytometry medicine.disease Up-Regulation ADAM Proteins Endocrinology chemistry Heart failure Ionomycin Leukocytes Mononuclear Female Tumor necrosis factor alpha medicine.symptom Cardiology and Cardiovascular Medicine business Biomarkers circulatory and respiratory physiology |
| Zdroj: | European Journal of Heart Failure. 6:869-875 |
| ISSN: | 1388-9842 |
| DOI: | 10.1016/j.ejheart.2004.02.007 |
| Popis: | Background: Tumor necrosis factor-α converting enzyme (TACE) has recently been identified as a metalloproteinase-disintegrin, which converts pro-tumor necrosis factor-α (TNF-α) to the mature form, and is an important mediator in the pathogenesis of CHF. Aims: In order to establish the importance of TACE in the regulation of TNF-α synthesis in peripheral blood mononuclear cells (PBMC), we analyzed mRNAs and protein-positive cells of both TACE and TNF-α in PBMC obtained from patients with congestive heart failure (CHF). Methods and results: PBMC were obtained from 46 patients with CHF and 22 controls. PBMC were activated by phorbol 12-myristate 13-acetate and ionomycin and assessed for TACE and TNF-α mRNAs by real-time RT-PCR, intracellular TACE and TNF-α levels by flow cytometry, and TNF-α secretion by supernatant ELISA. Levels of TACE and TNF-α mRNAs, intracellular TACE and TNF-α, and supernatant TNF-α were higher in CHF than in controls (P |
| Databáze: | OpenAIRE |
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