The Spore Coat Protein CotE Facilitates Host Colonization by Clostridium difficile
| Autor: | Jürgen Zentek, Siamand Hosseini, Huynh A. Hong, Saba Anwar, Simon M. Cutting, Anthony J. Wilkinson, William T. Ferreira, Mikhail Soloviev, Krisztina Hitri, Wilfried Vahjen |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
spores 030106 microbiology Colony Count Microbial Virulence Biology Host-Parasite Interactions Microbiology Mice Major Articles and Brief Reports 03 medical and health sciences Bacterial Proteins Intestinal mucosa Cell Wall mucus Cricetinae medicine Animals Immunology and Allergy Colonization Intestinal Mucosa Adhesins Bacterial Spores Bacterial Mesocricetus Bacteria Clostridioides difficile Chitinases Mucin fungi Mucins Peroxiredoxins Clostridium difficile colonization medicine.disease Mucus Spore Disease Models Animal Infectious Diseases chitinase Mutation Clostridium Infections Female Dysbiosis |
| Zdroj: | The Journal of Infectious Diseases |
| ISSN: | 0022-1899 |
| Popis: | Spores of Clostridium difficile carry a chitinase enzyme whose function is to bind to mucin, leading to its degradation. We show in vivo that this is potentially an important element of colonization and virulence. Clostridium difficile infection (CDI) is an important hospital-acquired infection resulting from the germination of spores in the intestine as a consequence of antibiotic-mediated dysbiosis of the gut microbiota. Key to this is CotE, a protein displayed on the spore surface and carrying 2 functional elements, an N-terminal peroxiredoxin and a C-terminal chitinase domain. Using isogenic mutants, we show in vitro and ex vivo that CotE enables binding of spores to mucus by direct interaction with mucin and contributes to its degradation. In animal models of CDI, we show that when CotE is absent, both colonization and virulence were markedly reduced. We demonstrate here that the attachment of spores to the intestine is essential in the development of CDI. Spores are usually regarded as biochemically dormant, but our findings demonstrate that rather than being simply agents of transmission and dissemination, spores directly contribute to the establishment and promotion of disease. |
| Databáze: | OpenAIRE |
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