Monitoring the intracellular transformation process of surface-cleavable PLGA particles containing disulfide bonds by fluorescence resonance energy transfer
| Autor: | Irina Estrela-Lopis, Guangyang Zou, Edwin Donath, Zhengwei Mao, Xiaojing Cui, Changyou Gao, Dahai Yu |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Biomedical Engineering General Chemistry General Medicine Polyethylene glycol chemistry.chemical_compound PLGA Förster resonance energy transfer chemistry Covalent bond Biophysics Organic chemistry Particle General Materials Science Bifunctional Fluorescein isothiocyanate Intracellular |
| Zdroj: | Journal of materials chemistry. B. 3(45) |
| ISSN: | 2050-7518 |
| Popis: | A great number of stimuli-responsive particles have been developed and used for biomedical applications such as intracellular drug delivery. It is of paramount importance to study the intracellular responsive process of these particles, offering insight into the understanding of their structure variation and design criteria for better performance. In this study polyethyleneimine (PEI)-coated poly(lactide-co-glycolide) (PLGA) particles with a diameter of 430 nm were prepared via a one-step emulsion method. The amino groups in the PEI molecules allowed further covalent linking of fluorescein isothiocyanate (FITC), bifunctional coupling agents 3,3′-dithiobispropionimidate, amino-ended polyethylene glycol (NH2–PEG–NH2) and tetramethylrhodamine isothiocyanate (TRITC), resulting in fluorescence resonance energy transfer (FRET) pairs on the particles. The particles exhibited glutathione-responsive ability, and lost the FRET effect due to the separation of FITC/TRITC pairs from the particle surface as a result of the cleavage of disulfide bonds. The particles showed different FRET change rates in A549 cells and HEK293 cells depending on the intracellular GSH concentration. Moreover, a much slower degradation rate was found inside cells than in simulated buffer with a similar GSH concentration. The results suggest that the responsive behaviors of the particles obtained in simulated buffer may not match fully/correctly with the real situation in a complicated intracellular environment. |
| Databáze: | OpenAIRE |
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