Deep functional immunophenotyping predicts risk of cytomegalovirus reactivation after hematopoietic cell transplantation
| Autor: | Eric D. Wieder, Krishna V. Komanduri, Deukwoo Kwon, Erik Kimble, Cara L. Benjamin, Jose F. Camargo, Xi Steven Chen, Despina Kolonias |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment Immunology Cytomegalovirus Hematopoietic stem cell transplantation CD8-Positive T-Lymphocytes Biochemistry Immunophenotyping Viral Matrix Proteins 03 medical and health sciences 0302 clinical medicine Risk Factors Aldesleukin medicine Humans Cumulative incidence Aged business.industry Hematopoietic Stem Cell Transplantation virus diseases Cell Biology Hematology Middle Aged Allografts Phosphoproteins Transplantation 030104 developmental biology 030220 oncology & carcinogenesis Cytomegalovirus Infections Cytokines Biomarker (medicine) Female Virus Activation Peptides business Biomarkers CD8 Ex vivo |
| Zdroj: | Blood. 133:867-877 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2018-10-878918 |
| Popis: | Cytomegalovirus (CMV) is the most common viral infection in hematopoietic cell transplantation (HCT) recipients. We performed deep phenotyping of CMV-specific T cells to predict CMV outcomes following allogeneic HCT. By using 13-color flow cytometry, we studied ex vivo CD8+ T-cell cytokine production in response to CMV-pp65 peptides in 3 clinically distinct subgroups of CMV-seropositive HCT patients: (1) Elite Controllers (n = 19): did not have evidence of CMV DNAemia on surveillance testing; (2) Spontaneous Controllers (n = 16): spontaneously resolved low-grade CMV DNAemia without antiviral therapy; and (3) Noncontrollers (NC; n = 21): experienced clinically significant CMV. Two CMV-specific CD8+ T-cell functional subsets were strongly associated with risk of CMV: (i) the nonprotective signature (NPS; IL-2−IFN-γ+TNF-α−MIP-1β+), found at increased levels among NC; and (ii) the protective signature (PS; IL-2+IFN-γ+TNF-α+MIP-1β+) found at low levels among NC. High levels of the NPS and low levels of PS were associated with an increased 100-day cumulative incidence of clinically significant CMV infection (35% vs 5%; P = .02; and 40% vs 12%; P = .05, respectively). The highest predictive value was observed when these signatures were combined into a composite biomarker consisting of low levels of the PS and high levels of the NPS (67% vs 10%; P < .001). After adjusting for steroid use or donor type, this composite biomarker remained associated with a fivefold increase in the risk of clinically significant CMV infection. CMV-specific CD8+ T-cell cytokine signatures with robust predictive value for risk of CMV reactivation should prove useful in guiding clinical decision making in HCT recipients. |
| Databáze: | OpenAIRE |
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