Progesterone withdrawal up-regulates fibronectin and integrins during menstruation and repair in the rhesus macaque endometrium
| Autor: | Rona S. Carroll, Ov D. Slayden, W. Cao, Robert M. Brenner, Kunie Mah |
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| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Integrins Ovariectomy Integrin Immunocytochemistry Uterus Gene Expression In situ hybridization Endometrium Internal medicine medicine Cell Adhesion Animals In Situ Hybridization Progesterone biology Estradiol Reverse Transcriptase Polymerase Chain Reaction Rehabilitation Obstetrics and Gynecology Immunohistochemistry Macaca mulatta Fibronectins Menstruation Up-Regulation Fibronectin medicine.anatomical_structure Endocrinology Reproductive Medicine In utero biology.protein Female Wound healing |
| Zdroj: | Human reproduction (Oxford, England). 22(12) |
| ISSN: | 0268-1161 |
| Popis: | BACKGROUND Fibronectin (FN) is a component of the extracellular matrix that participates in wound healing in various tissues as an adhesive ligand for integrins (Itgs). To determine whether these molecules play similar roles during menstrual repair, we evaluated the expression and localization of FN and specific Itgs in the primate endometrium under hormonally controlled conditions. METHODS Ovariectomized rhesus macaques were treated for 2 weeks with estradiol (E(2)) followed by E(2) with progesterone for 2 weeks. On day 28, progesterone was withdrawn and uteri were collected during menstruation, postmenstrual repair, and the proliferative and secretory phases. Analysis was by focused microarray, real time PCR, in situ hybridization and immunocytochemistry. RESULTS Progesterone withdrawal induced significant elevations of FN, Itg alpha5 and Itg beta1 transcripts during menstruation as compared to day 28 (FN: P < 0.01; Itg alpha5: P < 0.05; Itg beta1: P < 0.05; real time PCR). These increases were concentrated in the glandular epithelium (FN) and stroma (Itg alpha5beta1) of the uppermost zones. Cyclic changes in Itg alpha3 occurred in the glandular epithelium. CONCLUSIONS Spatially and temporally restricted peaks of expression of FN and its Itg receptors are closely correllated with menstruation and postmenstrual repair in the primate endometrium. |
| Databáze: | OpenAIRE |
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