A cell model system to study regulation of phosphotidylinositol 3-kinase and protein kinase B activity by cytokines/growth factors produced by type I collagen stimulated immune cells from patients with systemic sclerosis
| Autor: | Thomas M. Chiang, Arnold E. Postlethwaite |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
medicine.medical_specialty
Biophysics Enzyme-Linked Immunosorbent Assay Biology Biochemistry Peripheral blood mononuclear cell Article Collagen Type I Cell Line Arthritis Rheumatoid Phosphatidylinositol 3-Kinases Immune system Internal medicine Osteoarthritis medicine Humans Lupus Erythematosus Systemic Platelet Phosphorylation Molecular Biology Protein kinase B Cells Cultured Scleroderma Systemic Kinase Molecular biology Culture Media Endocrinology Cell culture Case-Control Studies Leukocytes Mononuclear Cytokines Intercellular Signaling Peptides and Proteins Tyrosine Nitric Oxide Synthase Megakaryocytes Protein Processing Post-Translational Proto-Oncogene Proteins c-akt Type I collagen |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1770:1181-1186 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2007.04.003 |
| Popis: | We have reported that posttranslational modification of systemic sclerosis patients' platelet phosphoinositide 1,3,4,5 kinase (PI 3-K) and protein kinase B (Akt) altered their enzymatic activities. In the present investigation, we have established a cell line model to study further the effects of posttranslational modification and modification by cytokines or growth factors of these two enzymes. Results from these studies suggest that posttranslational modification by phosphorylation of Akt and nitrotyrosylation of PI 3-K increases enzymatic activities, as was observed from SSc patients' platelets. These two signaling components are controlled by a different mechanism, which alters platelet reactivity towards the matrix components of vascular walls. We have used a megakaryotic cell line to study these two enzymes in the presence of cultured supernatants from peripheral blood mononuclear cells (PBMC), which were isolated from blood of SSc patients compared to controls including culture medium, rheumatoid arthritis, systemic lupus erythematosus, and osteoarthritis. The effect of the supernatants from SSc CI-stimulated PBMC cultures on both PI 3-K and Akt is specific. |
| Databáze: | OpenAIRE |
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