A mutant ankyrin protein kinase from Medicago sativa affects Arabidopsis adventitious roots
| Autor: | Pablo Rubén Gargantini, Marcela Raices, Florian Frugier, Veronica Giammaria, Delphine Chinchilla, Silvina Gonzalez-Rizzo, Rita M. Ulloa, Martin Crespi, Francisco L. Merchan |
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| Přispěvatelé: | Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, 'Dr. Héctor N. Torres' [Buenos Aires] (INGEBI), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET)-Facultad de Ciencias Exactas y Naturales [Buenos Aires] (FCEyN), Universidad de Buenos Aires [Buenos Aires] (UBA)-Universidad de Buenos Aires [Buenos Aires] (UBA), Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
0106 biological sciences
Mutant Plant Science 01 natural sciences Ciencias Biológicas 03 medical and health sciences Arabidopsis Botany Arabidopsis thaliana [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Medicago sativa Protein kinase A ComputingMilieux_MISCELLANEOUS 030304 developmental biology 2. Zero hunger 0303 health sciences biology Kinase fungi food and beverages ARABIDOPSIS THALIANA TUBULIN PHOSPHORYLATION [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Bioquímica y Biología Molecular biology.organism_classification Medicago truncatula Cell biology Agronomy and Crop Science Developmental biology CIENCIAS NATURALES Y EXACTAS 010606 plant biology & botany |
| Zdroj: | Functional Plant Biology Functional Plant Biology, CSIRO Publishing, 2008, 35 (1), ⟨10.1071/FP07209⟩ |
| ISSN: | 1445-4408 |
| DOI: | 10.1071/FP07209⟩ |
| Popis: | A family of plant kinases containing ankyrin-repeats, the Ankyrin-Protein Kinases (APKs), shows structural resemblance to mammalian Integrin-Linked Kinases (ILKs), key regulators of mammalian cell adhesion. MsAPK1 expression is induced by osmotic stress in roots of Medicago sativa (L.) plants. The Escherichia coli-purified MsAPK1 could only phosphorylate tubulin among a variety of substrates and the enzymatic activity was strictly dependent on Mn2+. MsAPK1 is highly related to two APK genes in Arabidopsis thaliana (L.), AtAPK1 and AtAPK2. Promoter-GUS fusions assays revealed that the Arabidopsis APK genes show distinct expression patterns in roots and hypocotyls. Although Medicago truncatula (L.) plants affected in MsAPK1 expression could not be obtained using in vitro regeneration, A. thaliana plants expressing MsAPK1 or a mutant MsAPK1 protein, in which the conserved aspartate 315 of the kinase catalytic domain was replaced by asparagines (DN-lines), developed normally. The DN mutant lines showed increased capacity to develop adventitious roots when compared with control or MsAPK1-expressing plants. APK-mediated signalling may therefore link perception of external abiotic signals and the microtubule cytoskeleton, and influence adventitious root development. Fil: Chinchilla, Delphine. Institut des Sciences du Végétal; Francia. Universidad de Basilea; Suiza Fil: Frugier, Florian. Institut des Sciences du Végétal; Francia Fil: Raices, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. The Salk Institute for Biological Studies; Estados Unidos Fil: Merchan, Francisco. Institut des Sciences du Végétal; Francia Fil: Giammaria, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Gargantini, Pablo Ruben. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina Fil: Gonzalez Rizzo, Silvina. Institut des Sciences du Végétal; Francia Fil: Crespi, Martin. Institut des Sciences du Végétal; Francia Fil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina |
| Databáze: | OpenAIRE |
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