Exploring the Role of AUG Triplets in Human Cap-Independent Translation Enhancing Elements
| Autor: | Amber N. Juba, John C. Chaput, Brian P. Wellensiek |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
RNA Caps Biochemistry & Molecular Biology Enhancer Elements 1.1 Normal biological development and functioning Cells Messenger Computational biology Biology Medical Biochemistry and Metabolomics Biochemistry Ribosome Article 03 medical and health sciences Medicinal and Biomolecular Chemistry Open Reading Frames Eukaryotic translation Methionine Genetic Underpinning research Protein biosynthesis Genetics Humans RNA Messenger Codon Cells Cultured Messenger RNA Cultured Base Sequence RNA Translation (biology) Ribosomal RNA 3. Good health Open reading frame 030104 developmental biology Enhancer Elements Genetic Hela Cells Protein Biosynthesis Mutation Generic health relevance Biochemistry and Cell Biology Ribosomes HeLa Cells |
| Zdroj: | Biochemistry Biochemistry, vol 57, iss 44 Juba, AN; Chaput, JC; & Wellensiek, BP. (2018). Exploring the Role of AUG Triplets in Human Cap-Independent Translation Enhancing Elements. BIOCHEMISTRY, 57(44), 6308-6318. doi: 10.1021/acs.biochem.8b00785. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/9t34p7m1 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/acs.biochem.8b00785. |
| Popis: | Cap-independent translation is believed to play an important role in eukaryotic protein synthesis, but the mechanisms of ribosomal recruitment and translation initiation remain largely unknown. Messenger RNA display was previously used to profile the human genome for RNA leader sequences that can enhance cap-independent translation. Surprisingly, many of the isolated sequences contain AUG triplets, suggesting a possible functional role for these motifs during translation initiation. Herein, we examine the sequence determinants of AUG triplets within a set of human translation enhancing elements (TEEs). Functional analyses performed in vitro and in cultured cells indicate that AUGs have the capacity to modulate mRNA translation either by serving as part of a larger ribosomal recruitment site or by directing the ribosome to defined initiation sites. These observations help constrain the functional role of AUG triplets in human TEEs and advance our understanding of this specific mechanism of cap-independent translation initiation. |
| Databáze: | OpenAIRE |
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