Decellularized skeletal muscles display neurotrophic effects in three-dimensional organotypic cultures
| Autor: | Silvia Perin, Paola Caccin, Paolo De Coppi, Mattia F. M. Gerli, Valentina Scattolini, Mei Hua Cui, Nicola Elvassore, Paolo Raffa, Camilla Luni, Anna Urciuolo |
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| Přispěvatelé: | Raffa P., Scattolini V., Gerli M.F.M., Perin S., Cui M., De Coppi P., Elvassore N., Caccin P., Luni C., Urciuolo A. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Nervous system Male Proteomics 3D culture axons decellularized muscle ECM innervation neurons organotypic culture spinal cord Animals Extracellular Matrix Female Humans Imaging Three-Dimensional Muscle Skeletal Rats Tissue Engineering Imaging Extracellular matrix CONSTRUCTS 0302 clinical medicine EXTRACELLULAR-MATRIX COMPONENTS PERIPHERAL-NERVE REPAIR Axon Tissue homeostasis proteomic axon lcsh:R5-920 Decellularization Chemistry ALLOGRAFTS lcsh:Cytology General Medicine Skeletal Cell biology medicine.anatomical_structure LAMINA Peripheral nervous system Muscle lcsh:Medicine (General) Life Sciences & Biomedicine Reinnervation 03 medical and health sciences Cell & Tissue Engineering REGENERATION Tissue Engineering and Regenerative Medicine medicine lcsh:QH573-671 Science & Technology GRAFT Skeletal muscle Cell Biology neuron 030104 developmental biology TISSUE Three-Dimensional 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine, Vol 9, Iss 10, Pp 1233-1243 (2020) Stem Cells Translational Medicine |
| Popis: | Skeletal muscle decellularization allows the generation of natural scaffolds that retain the extracellular matrix (ECM) mechanical integrity, biological activity, and three‐dimensional (3D) architecture of the native tissue. Recent reports showed that in vivo implantation of decellularized muscles supports muscle regeneration in volumetric muscle loss models, including nervous system and neuromuscular junctional homing. Since the nervous system plays pivotal roles during skeletal muscle regeneration and in tissue homeostasis, support of reinnervation is a crucial aspect to be considered. However, the effect of decellularized muscles on reinnervation and on neuronal axon growth has been poorly investigated. Here, we characterized residual protein composition of decellularized muscles by mass spectrometry and we show that scaffolds preserve structural proteins of the ECM of both skeletal muscle and peripheral nervous system. To investigate whether decellularized scaffolds could per se attract neural axons, organotypic sections of spinal cord were cultured three dimensionally in vitro, in presence or in absence of decellularized muscles. We found that neural axons extended from the spinal cord are attracted by the decellularized muscles and penetrate inside the scaffolds upon 3D coculture. These results demonstrate that decellularized scaffolds possess intrinsic neurotrophic properties, supporting their potential use for the treatment of clinical cases where extensive functional regeneration of the muscle is required. Decellularized muscles show direct neurotrophic properties. Here, we developed a three‐dimensional in vitro model in which organotypic sections of fetal spinal cord were cultured in presence or in absence of decellularized muscles. Decellularized muscles, which proteomic composition includes both myofibers and peripheral nerve components, sustained axon sprouting and attraction of neurons. |
| Databáze: | OpenAIRE |
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