A Composite Hydrogel Scaffold Permits Self‐Organization and Matrix Deposition by Cocultured Human Glomerular Cells
| Autor: | Jack Tuffin, Tim Johnson, Adam W. Perriman, Simon C. Satchell, Thomas O. Richardson, Gavin I. Welsh, Moin A. Saleem, Madeline Burke |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Collagen Type IV
kidneys Scaffold business.product_category Cell Survival Kidney Glomerulus Bristol Heart Institute Biomedical Engineering Pharmaceutical Science glomerulus 02 engineering and technology 010402 general chemistry 01 natural sciences Fibrin Biomaterials Imaging Three-Dimensional Tissue engineering Microfiber Cell Adhesion Journal Article medicine Humans Cell Proliferation Tissue Scaffolds biology Podocytes Chemistry Glomerular basement membrane Temperature Endothelial Cells Cell Differentiation Hydrogels Adhesion 021001 nanoscience & nanotechnology Coculture Techniques Extracellular Matrix 0104 chemical sciences medicine.anatomical_structure Cell culture Nanofiber scaffolds tissue engineering biology.protein Biophysics renal 0210 nano-technology business |
| Zdroj: | Tuffin, J, Burke, M, Richardson, T, Johnson, T, Saleem, M A, Satchell, S, Welsh, G I & Perriman, A 2019, ' A Composite Hydrogel Scaffold Permits Self-Organization and Matrix Deposition by Cocultured Human Glomerular Cells ', Advanced Healthcare Materials, vol. 8, no. 17, 1900698 . https://doi.org/10.1002/adhm.201900698 Tuffin, J, Burke, M, Richardson, T G, Johnson, T, Saleem, M, Satchell, S C, Welsh, G I & Perriman, A 2019, ' A Composite Hydrogel Scaffold Permits Self-Organization and Matrix Deposition by Cocultured Human Glomerular Cells ', Advanced Healthcare Materials . https://doi.org/10.1002/adhm.201900698 |
| Popis: | Three-dimensional scaffolds provide cells with a spatial environment that more closely resembles that of in vivo tissue, when compared to 2D culture on a plastic substrate. However, many scaffolding materials commonly used in tissue engineering tend to exhibit anisotropic morphologies that exhibit a narrow range of fibre diameters and pore-sizes, which do not recapitulate extracellular matrices. In this study, a fibrin hydrogel is formed within the interstitial spaces of an electrospun poly(glycolic) acid (PGA) monolith to generate a composite, bimodal scaffold for the co-culture of kidney glomerular cell lines. This new scaffold exhibits multiple fibre morphologies, containing both PGA microfibres (14.5 ± 2 µm) and fibrin gel nanofibres (0.14 ± 0.09 µm), which increase the compressive Young’s modulus beyond that of either of the constituents. The composite structure provides an enhanced 3D environment that increases proliferation and adhesion of immortalised human podocytes and glomerular endothelial cells. Moreover, the micro/nanoscale fibrous morphology promotes motility and reorganisation of the glomerular cells into glomerulus-like structures, resulting in the deposition of organised collagen IV; the primary component of the glomerular basement membrane (GBM). |
| Databáze: | OpenAIRE |
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