Matrix metalloproteinase 13 is a new contributor to skeletal muscle regeneration and critical for myoblast migration
| Autor: | Elisabeth R. Barton, Lucas R. Smith, Dephne Leong, Hanqin Lei |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Physiology Muscle Fibers Skeletal Cobra Cardiotoxin Proteins Gene Expression Matrix Metalloproteinase Inhibitors Biology Myoblasts Extracellular matrix Mice Myoblast fusion Cell Movement Matrix Metalloproteinase 13 medicine Animals Regeneration Myocyte Myoblast migration Muscle Skeletal Cells Cultured Regeneration (biology) Skeletal muscle Cell Differentiation Myoblast maturation Articles Cell Biology musculoskeletal system Cell biology Mice Inbred C57BL medicine.anatomical_structure Biochemistry C2C12 |
| Zdroj: | American Journal of Physiology-Cell Physiology. 305:C529-C538 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00051.2013 |
| Popis: | Efficient skeletal muscle repair and regeneration require coordinated remodeling of the extracellular matrix (ECM). Previous reports have indicated that matrix metalloproteinases (MMPs) play the pivotal role in ECM remodeling during muscle regeneration. The goal of the current study was to determine if the interstitial collagenase MMP-13 was involved in the muscle repair process. Using intramuscular cardiotoxin injections to induce acute muscle injury, we found that MMP-13 expression and activity transiently increased during the regeneration process. In addition, in muscles from mdx mice, which exhibit chronic injury, MMP-13 expression and protein levels were elevated. In differentiating C2C12 cells, a murine myoblast cell line, Mmp13 expression was most pronounced after myoblast fusion and during myotube formation. Using pharmacological inhibition of MMP-13 to test whether MMP-13 activity is necessary for the proliferation, differentiation, migration, and fusion of C2C12 cells, we found a dramatic blockade of myoblast migration, as well as a delay in differentiation. In contrast, C2C12 cells with stable overexpression of MMP-13 showed enhanced migration, without affecting myoblast maturation. Taken together, these results support a primary role for MMP-13 in myoblast migration that leads to secondary effects on differentiation. |
| Databáze: | OpenAIRE |
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