Proteins of human brain tissue obtained during surgical procedures
| Autor: | E. D. Ball, D. F. Cain, Anatole S. Dekaban |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adolescent Nerve Tissue Proteins Biology Astrocytoma Lipidoses Biochemistry White matter Cellular and Molecular Neuroscience chemistry.chemical_compound Cortex (anatomy) medicine Humans Child Gel electrophoresis Brain Chemistry Brain Diseases Epilepsy Isoelectric focusing Brain Neoplasms Histocytochemistry Brain Human brain Middle Aged Electrophoresis medicine.anatomical_structure Gliosis chemistry Solubility Acrylamide Electrophoresis Polyacrylamide Gel Female Subacute Sclerosing Panencephalitis medicine.symptom Isoelectric Focusing |
| Zdroj: | Journal of neurochemistry. 23(3) |
| ISSN: | 0022-3042 |
| Popis: | — A series of normal and abnormal brain tissues were obtained during surgical procedures; adjacent portions were evaluated histologically or extracted with dilute buffer. The proteins were separated in acrylamide gels using electrophoresis and isoelectric focusing. Twelve of the patients showed a very similar distribution of the proteins on electrophoretic separation referred to as the ‘typical’ pattern. This group includes all of the histologically normal specimens, and in addition, one mild gliosis, two cases of juvenile lipidosis, one mucopolysaccharidosis and one Unverricht epilepsy. Remarkably little variability in the electrophoresis gel pattern was found in a series of five pairs of histologically normal gray and white matter samples. An increased density of several bands was apparent in extracts of gray matter when compared with corresponding white. No differences which could be correlated with the different areas of the cortex were seen. Six of the patients showed gel patterns different from the ‘typical’ pattern. One of these (glioblastoma) differed only in the increase in band 9. Another glioblastoma showed a virtual absence of nearly all of the brain proteins usually found in the gels. Two astrocytomas and one surgical scar specimen showed a very dense protein band (No. 15) and a novel minor protein component, band 20, while one case of SSPE showed only the heavy band in the area of band 15. The content of band 5 was reduced in those specimens which presented a dense band 15. The pronounced increase in band 15 and band 20 appears to correlate with the marked increase in astrocytic elements in these three patients. |
| Databáze: | OpenAIRE |
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