Xyloglucan Metabolism Differentially Impacts the Cell Wall Characteristics of the Endosperm and Embryo during Arabidopsis Seed Germination
Autor: | Delphine Effroy-Cuzzi, Julien Sechet, Anne Frey, Delphine Charif, Grégory Mouille, François Perreau, Adeline Berger, Helen M. North, Loïc Rajjou, Gwendal Cueff, Annie Marion-Poll |
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Přispěvatelé: | Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, National Research Agency [ANR-2010-BLAN-1233-01, ANR-11-BSV5-0007] |
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Physiology [SDV]Life Sciences [q-bio] Mutant Arabidopsis Plant Science 01 natural sciences Hypocotyl Endosperm Cell Wall Gene Expression Regulation Plant Radicle Developmental Glucans Arabidopsis Proteins/genetics/metabolism Triazoles/pharmacology biology Gene Expression Regulation Developmental food and beverages Seeds/growth & development/metabolism Articles Plants Plants Genetically Modified Xylosidases Biochemistry Germination Seeds Glucans/*metabolism Xylans Xylosidases/genetics/metabolism Genetically Modified Genes Plant Arabidopsis/genetics/*growth & development/*metabolism Cell wall 03 medical and health sciences Genetics Cell Wall/metabolism Protein Processing Xylans/*metabolism Arabidopsis Proteins fungi Endosperm/growth & development/metabolism Post-Translational Plant Triazoles biology.organism_classification 030104 developmental biology Genes Gene Expression Regulation Germination/drug effects/genetics/physiology Mutation Dormancy abscisic-acid monoclonal-antibodies aba accumulation gene-expression growth defects thaliana seeds messenger-rna dormancy mutant deficient Protein Processing Post-Translational 010606 plant biology & botany |
Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2016, 170 (3), pp.1367--80. ⟨10.1104/pp.15.01312⟩ Plant Physiology, American Society of Plant Biologists, 2016, 170, pp.1367--80. ⟨10.1104/pp.15.01312⟩ Plant Physiology 3 (170), 1367-1380. (2016) |
ISSN: | 0032-0889 1532-2548 |
DOI: | 10.1104/pp.15.01312⟩ |
Popis: | International audience; Cell wall remodeling is an essential mechanism for the regulation of plant growth and architecture, and xyloglucans (XyGs), the major hemicellulose, are often considered as spacers of cellulose microfibrils during growth. In the seed, the activity of cell wall enzymes plays a critical role in germination by enabling embryo cell expansion leading to radicle protrusion, as well as endosperm weakening prior to its rupture. A screen for Arabidopsis (Arabidopsis thaliana) mutants affected in the hormonal control of germination identified a mutant, xyl1, able to germinate on paclobutrazol, an inhibitor of gibberellin biosynthesis. This mutant also exhibited reduced dormancy and increased resistance to high temperature. The XYL1 locus encodes an alpha-xylosidase required for XyG maturation through the trimming of Xyl. The xyl1 mutant phenotypes were associated with modifications to endosperm cell wall composition that likely impact on its resistance, as further demonstrated by the restoration of normal germination characteristics by endosperm-specific XYL1 expression. The absence of phenotypes in mutants defective for other glycosidases, which trim Gal or Fuc, suggests that XYL1 plays the major role in this process. Finally, the decreased XyG abundance in hypocotyl longitudinal cell walls of germinating embryos indicates a potential role in cell wall loosening and anisotropic growth together with pectin de-methylesterification. |
Databáze: | OpenAIRE |
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