Xyloglucan Metabolism Differentially Impacts the Cell Wall Characteristics of the Endosperm and Embryo during Arabidopsis Seed Germination

Autor: Delphine Effroy-Cuzzi, Julien Sechet, Anne Frey, Delphine Charif, Grégory Mouille, François Perreau, Adeline Berger, Helen M. North, Loïc Rajjou, Gwendal Cueff, Annie Marion-Poll
Přispěvatelé: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, National Research Agency [ANR-2010-BLAN-1233-01, ANR-11-BSV5-0007]
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
[SDV]Life Sciences [q-bio]
Mutant
Arabidopsis
Plant Science
01 natural sciences
Hypocotyl
Endosperm
Cell Wall
Gene Expression Regulation
Plant

Radicle
Developmental
Glucans
Arabidopsis Proteins/genetics/metabolism
Triazoles/pharmacology
biology
Gene Expression Regulation
Developmental

food and beverages
Seeds/growth & development/metabolism
Articles
Plants
Plants
Genetically Modified

Xylosidases
Biochemistry
Germination
Seeds
Glucans/*metabolism
Xylans
Xylosidases/genetics/metabolism
Genetically Modified
Genes
Plant

Arabidopsis/genetics/*growth & development/*metabolism
Cell wall
03 medical and health sciences
Genetics
Cell Wall/metabolism
Protein Processing
Xylans/*metabolism
Arabidopsis Proteins
fungi
Endosperm/growth & development/metabolism
Post-Translational
Plant
Triazoles
biology.organism_classification
030104 developmental biology
Genes
Gene Expression Regulation
Germination/drug effects/genetics/physiology
Mutation
Dormancy
abscisic-acid
monoclonal-antibodies
aba accumulation
gene-expression
growth defects
thaliana seeds
messenger-rna
dormancy
mutant
deficient
Protein Processing
Post-Translational

010606 plant biology & botany
Zdroj: Plant Physiology
Plant Physiology, American Society of Plant Biologists, 2016, 170 (3), pp.1367--80. ⟨10.1104/pp.15.01312⟩
Plant Physiology, American Society of Plant Biologists, 2016, 170, pp.1367--80. ⟨10.1104/pp.15.01312⟩
Plant Physiology 3 (170), 1367-1380. (2016)
ISSN: 0032-0889
1532-2548
DOI: 10.1104/pp.15.01312⟩
Popis: International audience; Cell wall remodeling is an essential mechanism for the regulation of plant growth and architecture, and xyloglucans (XyGs), the major hemicellulose, are often considered as spacers of cellulose microfibrils during growth. In the seed, the activity of cell wall enzymes plays a critical role in germination by enabling embryo cell expansion leading to radicle protrusion, as well as endosperm weakening prior to its rupture. A screen for Arabidopsis (Arabidopsis thaliana) mutants affected in the hormonal control of germination identified a mutant, xyl1, able to germinate on paclobutrazol, an inhibitor of gibberellin biosynthesis. This mutant also exhibited reduced dormancy and increased resistance to high temperature. The XYL1 locus encodes an alpha-xylosidase required for XyG maturation through the trimming of Xyl. The xyl1 mutant phenotypes were associated with modifications to endosperm cell wall composition that likely impact on its resistance, as further demonstrated by the restoration of normal germination characteristics by endosperm-specific XYL1 expression. The absence of phenotypes in mutants defective for other glycosidases, which trim Gal or Fuc, suggests that XYL1 plays the major role in this process. Finally, the decreased XyG abundance in hypocotyl longitudinal cell walls of germinating embryos indicates a potential role in cell wall loosening and anisotropic growth together with pectin de-methylesterification.
Databáze: OpenAIRE
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