The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles
| Autor: | M. J. Browning, J. A. Madrigal, P. Krausa, H. Kowalski, C. E. M. Allsopp, A-M. Little, S. Turner, E. J. Adams, K. L. Arnett, W. F. Bodmer, J. G. Bodmer, P. Parham |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Molecular Sequence Data
Immunology Black People Genes MHC Class I HLA-C Antigens Human leukocyte antigen Biology Polymerase Chain Reaction Biochemistry DNA sequencing Exon Gene Frequency Antigen Genotype Tumor Cells Cultured Genetics Humans Immunology and Allergy Allele Alleles Base Sequence HLA-A Antigens DNA General Medicine Africa Eastern Molecular biology Subtyping HLA-A HLA-B Antigens |
| Zdroj: | Scopus-Elsevier |
| Popis: | Daudi, a lymphoblastoid B cell line derived from an African Burkitt lymphoma does not express HLA-A,B,C antigens at the cell surface. Although HLA-A,B,C heavy chains are made normally they do not assemble into functional molecules because beta 2-microglobulin is absent. Previous serological analysis of somatic cell hybrids indicated that the HLA haplotypes of Daudi encoded HLA-A1, A10(A26), B17, and B16(38) antigens. Here we describe the application of molecular methods: ARMS-PCR, cDNA cloning and sequencing, immunoprecipitation and gel electrophoresis, to define the class I genotype of the Daudi cell line which is HLA-A*0102, A*6601, B*5801, B*5802, Cw*0302 and Cw*0602. With the exception of the B38 antigen, which is not a product of the alleles defined, the genotype is consistent with the serological description. Two previously undiscovered alleles emerged from this analysis: A*0102 and B*5802. The A*0102 allele differs from A*0101 by 5 nucleotide substitutions within exon 2 where it has a motif shared with A*30 alleles; the B*5802 allele differs from B*5801 by 3 substitutions in exon 3 where it has a motif shared with B*14 alleles. Subtyping HLA-A1 alleles showed A*0102 was well represented amongst individuals typed serologically as A1 in an African population but was absent from caucasoids. B*5802 has been found in a second individual. Thus the novel A and B alleles are not specific to the Daudi tumor. Overall, this analysis of a single East African cell illustrates the power of molecular methods to define new class I HLA alleles in non-caucasoid populations. |
| Databáze: | OpenAIRE |
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