Protein kinase A signalling via CREB controls myogenesis induced by Wnt proteins
Autor: | Chen-Ming Fan, Alice E. Chen, David D. Ginty |
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Rok vydání: | 2004 |
Předmět: |
Muscle Proteins
CREB MyoD Muscle Development Cell Line Embryo Culture Techniques Mice Animals Paired Box Transcription Factors RNA Messenger Protein kinase A Cyclic AMP Response Element-Binding Protein Transcription factor PAX3 Transcription Factor MyoD Protein Mice Knockout Multidisciplinary biology Myogenesis Muscles Wnt signaling pathway Gene Expression Regulation Developmental Cyclic AMP-Dependent Protein Kinases Coculture Techniques DNA-Binding Proteins Wnt Proteins Somites Mutation Cancer research biology.protein Trans-Activators Intercellular Signaling Peptides and Proteins MYF5 Myogenic Regulatory Factor 5 Signal transduction Adenylyl Cyclases Signal Transduction Transcription Factors |
Zdroj: | Nature. 433(7023) |
ISSN: | 1476-4687 |
Popis: | Select members of the Wnt family of secreted glycoproteins have been implicated in inducing the myogenic determinant genes Pax3, MyoD and Myf5 during mammalian embryogenesis, but the mechanism of induction has not been defined. We describe an unexpected role for protein kinase A (PKA) signalling via CREB in this induction. Using a combination of in vitro explant assays, mutant analysis and gene delivery into mouse embryos cultured ex vivo, we demonstrate that adenylyl cyclase signalling via PKA and its target transcription factor CREB are required for Wnt-directed myogenic gene expression. Wnt proteins can also stimulate CREB-mediated transcription, providing evidence for a Wnt signalling pathway involving PKA and CREB. Our findings raise the possibility that PKA/CREB signalling may also contribute to other Wnt-regulated processes in embryonic patterning, stem cell renewal and cancer. |
Databáze: | OpenAIRE |
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