Synchronized Optical and Electronic Detection of Biomolecules Using a Low Noise Nanopore Platform
| Autor: | Ki-Bum Kim, Jae-Seok Yu, Tim Albrecht, Robin J. Leatherbarrow, Hyung-Jun Kim, William H. Pitchford, Hyun-Mi Kim, Joshua B. Edel, Aleksandar P. Ivanov |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
Optical Phenomena Silicon General Physics and Astronomy chemistry.chemical_element Nanotechnology 02 engineering and technology Substrate (electronics) Signal-To-Noise Ratio 010402 general chemistry 01 natural sciences Capacitance Noise (electronics) Fluorescence spectroscopy Potassium Chloride Nanopores Electricity General Materials Science chemistry.chemical_classification Biomolecule Silicon Compounds Electric Conductivity General Engineering Membranes Artificial DNA 021001 nanoscience & nanotechnology QR 0104 chemical sciences Optical phenomena Nanopore chemistry 0210 nano-technology |
| Zdroj: | ACS Nano |
| ISSN: | 1936-0851 |
| DOI: | 10.1021/nn506572r |
| Popis: | In the past two decades there has been a tremendous amount of research into the use of nanopores as single molecule sensors, which has been inspired by the Coulter counter and molecular transport across biological pores. Recently, the desire to increase structural resolution and analytical throughput has led to the integration of additional detection methods such as fluorescence spectroscopy. For structural information to be probed electronically high bandwidth measurements are crucial due to the high translocation velocity of molecules. The most commonly used solid-state nanopore sensors consist of a silicon nitride membrane and bulk silicon substrate. Unfortunately, the photoinduced noise associated with illumination of these platforms limits their applicability to high-bandwidth, high-laser-power synchronized optical and electronic measurements. Here we present a unique low-noise nanopore platform, composed of a predominately Pyrex substrate and silicon nitride membrane, for synchronized optical and electronic detection of biomolecules. Proof of principle experiments are conducted showing that the Pyrex substrates have substantially lowers ionic current noise arising from both laser illumination and platform capacitance. Furthermore, using confocal microscopy and a partially metallic pore we demonstrate high signal-to-noise synchronized optical and electronic detection of dsDNA. |
| Databáze: | OpenAIRE |
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