Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry
Autor: | Mette Olaf Nielsen, Andrew J. Cole, Annette Bruhn, Natalja P. Nørskov |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
food.ingredient Endocrinology Diabetes and Metabolism phenolics Flavonoid Gas Chromatography-Mass Spectrometry (GC-MS) 01 natural sciences Biochemistry Microbiology Article 03 medical and health sciences chemistry.chemical_compound Metabolomics food Liquid chromatography–mass spectrometry Asparagopsis metabolomic profiling Molecular Biology chemistry.chemical_classification Chromatography biology methane 010401 analytical chemistry Liquid Chromatography-Mass Spectrometry (LC-MS) hemp biology.organism_classification greenhouse gasses QR1-502 0104 chemical sciences seaweeds halomethanes 030104 developmental biology chemistry Sargassum Asparagopsis taxiformis Gas chromatography Bromoform |
Zdroj: | Metabolites, Vol 11, Iss 259, p 259 (2021) Nørskov, N, Bruhn, A, Cole, A & Nielsen, M O 2021, ' Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry ', Metabolites, vol. 11, no. 5, 259 . https://doi.org/10.3390/metabo11050259 Metabolites Metabolites; Volume 11; Issue 5; Pages: 259 |
ISSN: | 2218-1989 |
Popis: | Greenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds are theorized to reduce enteric methane emissions. In this study we aimed to investigate the metabolic profiles of brown, red and green seaweeds and hemp using gas chromatography and liquid chromatography mass spectrometry. We used targeted and untargeted approaches, quantifying known halomethanes and phenolics, as well as identifying potentially novel bioactive compounds with anti-methanogenic properties. The main findings were: (a) Asparagopsis taxiformis contained halomethanes, with high concentrations of bromoform (4200 µg/g DW), six volatile halocarbons were tentatively identified; (b) no halomethanes were detected in the other studied seaweeds nor in hemp; (c) high concentrations of lignans were measured in hemp; (d) a high numbers of sulfated phenolic acids and unidentified sulfuric acid-containing compounds were detected in all seaweeds; (e) flavonoid glucosides and glucuronides were mainly identified in hemp; and (f) the condensed tannin gallocatechin was tentatively identified in Fucus sp. Using the combined metabolomics approach, an overview and in-depth information on secondary metabolites were provided. Halomethanes of Asparagopsis sp. have already been shown to be anti-methanogenic; however, metabolic profiles of seaweeds such as Dictyota and Sargassum have also been shown to contain compounds that may have anti-methanogenic potential. |
Databáze: | OpenAIRE |
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