Isolation and characterization of prosthecae ofAsticcacaulis biprosthecum
| Autor: | T. L. Jordan, J. S. Porter, Jack L. Pate |
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| Rok vydání: | 1974 |
| Předmět: |
DNA
Bacterial Cell Carbohydrates Dehydrogenase Glucosephosphate Dehydrogenase Reductase Biology Biochemistry Microbiology chemistry.chemical_compound Prosthecate bacteria Bacterial Proteins Cell Wall Malate Dehydrogenase Genetics medicine NADH NADPH Oxidoreductases Centrifugation Molecular Biology chemistry.chemical_classification Growth medium Bacteria Cell-Free System L-Lactate Dehydrogenase General Medicine Alkaline Phosphatase Electrophoresis Disc Lipids Molecular biology Isocitrate Dehydrogenase Microscopy Electron RNA Bacterial medicine.anatomical_structure Enzyme chemistry Alkaline phosphatase |
| Zdroj: | Archives of Microbiology. 96:1-16 |
| ISSN: | 1432-072X 0302-8933 |
| Popis: | Prosthecae were sheared from cells ofAsticcacaulis biprosthecum with a Sorvall omnimixer and purified by centrifugation. Removal of prosthecae resulted in no loss in viability. Purified prosthecae contain protein, carbohydrates and lipids. Certain enzymes that are present in the main body of the cell are also present in prosthecae: malic dehydrogenase (E.C. 1.1.1.37), alkaline phosphatase (E. C. 3.1.3.1), succinic dehydrogenase (E.C. 1.3.99.1), cytochromec reductase (E.C. 1.6.2.a), and cytochromes. Other enzymes present in whole cells are not in prosthecae: isocitric dehydrogenase (E.C. 1.1.1.41), NADH oxidase (E.C. 1.6.99.3), glucose-6-phosphate dehydrogenase (E.C. 1.1.1.49), and lactic dehydrogenase (E.C. 1.1.2.3). Swarmer cells (non-prosthecate) were purified by allowing prosthecate cells to attach to cheesecloth in the growth medium. Envelopes prepared from prosthecae were compared with envelope from swarmer cells. The two envelope fractions differed in the disc-gel electrophoretic patterns of proteins solubilized from them. |
| Databáze: | OpenAIRE |
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