Encoding of cutaneous stimuli by lamina I projection neurons
| Autor: | Kim I. Chisholm, Stephen B. McMahon, Andrew J. Todd, Laure Lo Re, Maria Gutierrez-Mecinas, Erika Polgár |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Dorsum
Nociception Lamina Spinal Cord Dorsal Horn Pain Biology Interneurons medicine Genetically encoded calcium indicators Projection (set theory) Skin Spinal cord Dorsal horn Cold Temperature Electrophysiology Anesthesiology and Pain Medicine medicine.anatomical_structure Neurology Confocal Neurology (clinical) Neuroscience Thermal sensation Research Paper |
| Zdroj: | Pain |
| ISSN: | 1872-6623 0304-3959 |
| Popis: | Supplemental Digital Content is Available in the Text. We here report on the use of in vivo optical imaging to study lamina I projection neurons and their response to cutaneous stimuli. Lamina I of the dorsal horn, together with its main output pathway, lamina I projection neurons, has long been implicated in the processing of nociceptive stimuli, as well as the development of chronic pain conditions. However, the study of lamina I projection neurons is hampered by technical challenges, including the low throughput and selection biases of traditional electrophysiological techniques. Here we report on a technique that uses anatomical labelling strategies and in vivo imaging to simultaneously study a network of lamina I projection neurons in response to electrical and natural stimuli. Although we were able to confirm the nociceptive involvement of this group of cells, we also describe an unexpected preference for innocuous cooling stimuli. We were able to characterize the thermal responsiveness of these cells in detail and found cooling responses decline when exposed to stable cold temperatures maintained for more than a few seconds, as well as to encode the intensity of the end temperature, while heating responses showed an unexpected reliance on adaptation temperatures. |
| Databáze: | OpenAIRE |
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