Polyphenolic Content and Physiological Activities of Chinese Hawthorn Extracts
| Autor: | Yi-Ling Tian, Kozo Nakamura, Tong Cui, Su Tian, Hiroshi Kayahara |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Antioxidant medicine.medical_treatment Ethyl acetate Applied Microbiology and Biotechnology Biochemistry Antioxidants Crataegus Analytical Chemistry Chinese hawthorn chemistry.chemical_compound Phenols Chlorogenic acid medicine Enzyme Inhibitors Molecular Biology Chromatography High Pressure Liquid Flavonoids Chromatography biology Plant Extracts Chemistry Organic Chemistry Polyphenols food and beverages General Medicine biology.organism_classification Crataegus pinnatifida Proanthocyanidin Polyphenol Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 70:2948-2956 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.60361 |
| Popis: | Hawthorn polyphenol (HP) was prepared by ethyl acetate treatment of the ethanol extract (HE) of Chinese hawthorn fruit. The concentrations of 15 polyphenols in the HP, HE, extraction residue (HJ), and a hawthorn leaf extract (HF) were determined by HPLC. For HP, the total content of the 15 polyphenols was 21.4%, comprised of 19.7% of procyanidins, 1.21% of chlorogenic acid, and 0.48% of flavonoids, compared to 2.55% for the HE. The yields of procyanidin monomer, dimer, trimer, tetramer, and pentamer were 50.5%, 30.3%, 23.0%, 14.6%, and 12.5% respectively, and the mean degree of polymerization was reduced to 1.39 (HP) from 1.65 (HE). Seven different physiological actions of the four extracts were investigated. The HP showed strong O(2)(-) and (.-)OH scavenging capacities (IC(50) values of 6.3 microg/ml and 1.1 microg/ml respectively), as well as selective prolyl endopeptidase inhibition (IC(50) of 60 microg/ml). The active constituents appeared to be procyanidins. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|