Regulation of MMP 2 and MMP 9 expressions modulated by AP-1 (c-jun) in wound healing: improving role of Lucilia sericata in diabetic rats
| Autor: | Gonul Kanigur-Sultuybek, Elif Yaprak Sarac, Ender Coskunpinar, Matem Tunçdemir, Serhat Sirekbasan, Fatma Kubra Tombulturk, Erdal Polat, Tugba Soydas |
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| Přispěvatelé: | İstinye Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Bölümü, Fatma Kübra Tombultürk / 0000-0002-4358-2309, Tombultürk, Fatma Kübra, Fatma Kübra Tombultürk / L-3969-2018, Fatma Kübra Tombultürk / 56748052500 |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Endocrinology Diabetes and Metabolism Maggot Debridement Therapy 030209 endocrinology & metabolism Inflammation 030204 cardiovascular system & hematology Matrix metalloproteinase Lucilia Sericata Ap-1 Diabetes Mellitus Experimental Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Diabetes mellitus Internal Medicine Animals Medicine Wound Healing Stz Diabetes P53 Mmp business.industry Activator (genetics) Diptera c-jun General Medicine Fibroblasts medicine.disease Streptozotocin Diabetic Foot Rats Transcription Factor AP-1 Treatment Outcome Matrix Metalloproteinase 9 Larva Insect Proteins Matrix Metalloproteinase 2 Immunohistochemistry medicine.symptom business Wound healing Signal Transduction medicine.drug |
| Popis: | Sirekbasan, Serhat/0000-0001-7967-3539; Tuncdemir, Matem/0000-0002-5300-4449; Tombulturk, Fatma Kubra/0000-0002-4358-2309 AimsLucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1. c-jun has been demonstrated to be a repressor of p53 in immortalized fibroblasts. The aim of the present study is to investigate the effects of L. sericata ES on the expression of AP-1 (c-jun), p53, MMP-2, and MMP-9 in wound biopsies dissected from streptozotocin induced diabetic rats.MethodsThe expression levels of MMP-2, MMP-9, c-jun and p53 in dermal tissues were determined at days 0, 3, 7 and 14 after wounding, using immunohistochemical analysis and quantitative real-time PCR.ResultsThe treatment with ES significantly decreased through inflammation-based induction of MMP-2 and MMP-9 expression levels in the wounds of diabetic groups, compared to control groups at the third day of wound healing. At the 14th day, there were dramatic decreases in expression of c-jun, MMP-9, and p53 in ES-treated groups, compared to the diabetic group (P |
| Databáze: | OpenAIRE |
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