NOD mice have a severly impaired ability to recruit leukocytes into sites of inflammation
| Autor: | Pieter J. M. Leenen, Cornelia G van Helden-Meeuwsen, Tatjana Nikolic, Gerben Bouma, Jojanneke M. C. Coppens, Silvano Sozzani, Hemmo A. Drexhage, Marjan A. Versnel |
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| Přispěvatelé: | Immunology |
| Rok vydání: | 2005 |
| Předmět: |
Chemokine
Immunology Inflammation Nod Biology CCL2 In Vitro Techniques Monocytes Peritoneal cavity Mice Peritoneum Cell Movement Mice Inbred NOD medicine Leukocytes Immunology and Allergy Animals Peritoneal Cavity Chemokine CCL2 NOD mice Mice Inbred BALB C Macrophages hemic and immune systems Dendritic Cells Immunity Innate Interleukin-10 Mice Inbred C57BL Interleukin 10 medicine.anatomical_structure Diabetes Mellitus Type 1 biology.protein Female medicine.symptom |
| Zdroj: | European Journal of Immunology, 35(1), 225-235. Wiley-VCH |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/eji.200425513 |
| Popis: | The accumulation of macrophages (M Phi) and dendritic cells (DC) in the pancreas plays a crucial role in the pathogenesis of autoimmune diabetes. We studied the recruitment of monocytes, M Phi and DC to sites of inflammation, i.e. the peritoneal cavity and a subcutaneously elicited air pouch in the NOD mouse model of autoimmune diabetes. The leukocyte recruitment was studied from 1 to 7 days after injection of thioglycollate (peritoneum), C5a (peritoneum, air pouch), CCL2 and CCL3 (air pouch). C57BL/6 and BALB/c mice served as controls. Morphological and flow cytometric analysis of the recruited cells was performed, IL-1 beta, TNF-alpha, IL-6, IL-12 and IL-10 in exudates measured, and in vitro CCL2-chemotaxis of exudate M Phi (Boyden chamber) determined. NOD mice were strongly impaired in the recruitment of M Phi, DC, monocytes, and granulocytes. Chemokine-injected air pouches of NOD mice showed an increased IL-10 and a decreased IL-1 beta level, while the other cytokines were normally or very lowly expressed. In addition, NOD exudate M Phi displayed an impaired in vitro CCL2-induced migration. Our data show that NOD mice have an impaired ability to recruit leukocytes into sites of inflammation elicited in the peritoneum and the air pouch. A raised IL-10/IL-1 beta ratio at these sites and a deficient migratory capacity of NOD monocytes are important determinants in this impairment. |
| Databáze: | OpenAIRE |
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