Bioprinting Living Biofilms through Optogenetic Manipulation
| Autor: | Yajia Huang, Aiguo Xia, Guang Yang, Fan Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Light Computer science Bioprinting Biomedical Engineering Biofilm Nanotechnology General Medicine Optogenetics Biochemistry Genetics and Molecular Biology (miscellaneous) Microprinting 03 medical and health sciences 030104 developmental biology Biofilms Pseudomonas aeruginosa High spatial resolution Microorganisms Genetically-Modified Cyclic GMP |
| Zdroj: | ACS Synthetic Biology. 7:1195-1200 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.8b00003 |
| Popis: | In this paper, we present a new strategy for microprinting dense bacterial communities with a prescribed organization on a substrate. Unlike conventional bioprinting techniques that require bioinks, through optogenetic manipulation, we directly manipulated the behaviors of Pseudomonas aeruginosa to allow these living bacteria to autonomically form patterned biofilms following prescribed illumination. The results showed that through optogenetic manipulation, patterned bacterial communities with high spatial resolution (approximately 10 μm) could be constructed in 6 h. Thus, optogenetic manipulation greatly increases the range of available bioprinting techniques. |
| Databáze: | OpenAIRE |
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