Post-operative immune suppression is mediated via reversible, Interleukin-10 dependent pathways in circulating monocytes following major abdominal surgery
| Autor: | Hew D.T. Torrance, Rupert M Pearse, Michael J. O’Dwyer, Charles J. Hinds, Bagrat Lalabekyan, Tom E.F. Abbott, Gareth L. Ackland, Mark Vivian, E. Rebecca Longbottom |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine education lcsh:Medicine Monocytes Interferon-gamma 03 medical and health sciences Postoperative Complications 0302 clinical medicine Immune system Risk Factors Abdomen Immune Tolerance medicine Humans RNA Messenger Post operative lcsh:Science Cells Cultured Digestive System Surgical Procedures health care economics and organizations Aged Multidisciplinary business.industry lcsh:R Granulocyte-Macrophage Colony-Stimulating Factor 030208 emergency & critical care medicine HLA-DR Antigens Middle Aged Interleukin-10 Interleukin 10 surgical procedures operative 030104 developmental biology medicine.anatomical_structure Elective Surgical Procedures Anesthesia Abdomen surgery Female lcsh:Q business Abdominal surgery |
| Zdroj: | PLoS ONE, Vol 13, Iss 9, p e0203795 (2018) |
| ISSN: | 1932-6203 |
| Popis: | INTRODUCTION:Post-operative infections occur frequently following major surgery. The magnitude of the post-operative immune response is associated with an increased risk of post-operative infections, although the mechanisms driving post-operative immune-dysfunction and the potential reversibility of this response with immune stimulants are not well understood. This study aims to describe the immediate immune response to major surgery and establish links to both post-operative infection and functional aspects of immune dysregulation. We also investigate the potential of clinically available immune stimulants to reverse features of post-operative immune-dysfunction. METHODS:Patients over 45 years old undergoing elective gastro-intestinal surgery with planned post-operative surgical ICU admission were recruited. The expression of selected genes was determined pre-operatively and at 2, 24 and 48 hours post-operatively using qRT-PCR. Circulating levels of Interleukin-10 protein were determined by ELISA. Peri-operative cell surface monocyte HLA-DR (mHLA-DR) expression was determined using flow cytometry. Gene expression and mHLA-DR levels were determined in healthy monocytes cultured in peri-operative serum with and without neutralising antibodies and immune stimulants. RESULTS:119 patients were recruited; 44 developed a post-operative infection. Interleukin-10 mRNA and protein increased 4-fold post-operatively (P |
| Databáze: | OpenAIRE |
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