Cytotoxic activity of standardized extracts, a fraction, and individual secondary metabolites from fenugreek seeds against SKOV-3, HeLa and MOLT-4 cell lines
Autor: | Katarzyna Kimel, Barbara Sparzak-Stefanowska, Justyna Stefanowicz-Hajduk, Mirosława Krauze-Baranowska, J. Renata Ochocka, Barbara Król-Kogus |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Pharmaceutical Science
Secondary Metabolism Uterine Cervical Neoplasms 030226 pharmacology & pharmacy 01 natural sciences HeLa 0302 clinical medicine Drug Discovery Cytotoxic T cell HaCaT Cells Chromatography High Pressure Liquid Membrane Potential Mitochondrial Ovarian Neoplasms steroidal saponins biology Chemistry Drug Synergism General Medicine rtca trigonella foenum-graecum Biochemistry Seeds Molecular Medicine Female Research Article musculoskeletal diseases Trigonella Fraction (chemistry) RM1-950 03 medical and health sciences Inhibitory Concentration 50 Cell Line Tumor Humans Pharmacology Flavonoids Plant Extracts flavone c-glycosides Fabaceae Saponins biology.organism_classification Antineoplastic Agents Phytogenic 0104 chemical sciences Leukemia Lymphoid 010404 medicinal & biomolecular chemistry Complementary and alternative medicine Cell culture Cancer cell cancer cells Therapeutics. Pharmacology Reactive Oxygen Species HeLa Cells |
Zdroj: | Pharmaceutical Biology, Vol 59, Iss 1, Pp 424-437 (2021) Pharmaceutical Biology article-version (VoR) Version of Record |
ISSN: | 1744-5116 1388-0209 |
Popis: | Context Trigonella foenum-graecum L. (Fabaceae) has many therapeutic properties and anticancer potential. Objective The cytotoxic activities of standardized extracts and a fraction from fenugreek seeds and their compounds (sapogenins, flavone C-glycosides, alkaloid trigonelline) against human cancer SKOV-3, HeLa and MOLT-4 cells were evaluated. Materials and methods Fenugreek seeds were extracted with 70% methanol (A) or water (B). Furthermore, the seeds were purified with petroleum ether and chloroform and next extracted with methanol to obtain fraction (C). The quantitative analysis of saponins and flavonoids in the extracts was done with HPLC methods. The extracts (5–120 µg/mL) and compounds (1–50 µg/mL) were tested on the cells by MTT assay and RTCA system. The effect of a fraction on ROS production, mitochondrial membrane potential and caspase-3/7 activity in HeLa and SKOV-3 cells was also evaluated by flow cytometry. Results The strongest cytotoxic activity on cancer cells showed the fraction C (IC50 was 3.91 ± 0.03 for HeLa, 3.97 ± 0.07 for SKOV-3, and 7.75 ± 0.37 for MOLT-4) with the highest content of steroidal saponins (163.18 ± 11.03 μg/mg) and flavone C-glycosides (820.18 ± 0.05 μg/mg). The fraction significantly increased ROS production (up to four times higher than in keratinocytes as control) and caspases activity in the cells. The examined flavonoids did not exhibit the cytotoxic activity in contrast to yamogenin, tigogenin, and diosgenin. Conclusions The obtained results complement the data on the cytotoxic activity of Foenugraeci Semen and synergistic effect of flavonoids and saponins complex contained in the plant. |
Databáze: | OpenAIRE |
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