Cytotoxic activity of standardized extracts, a fraction, and individual secondary metabolites from fenugreek seeds against SKOV-3, HeLa and MOLT-4 cell lines

Autor: Katarzyna Kimel, Barbara Sparzak-Stefanowska, Justyna Stefanowicz-Hajduk, Mirosława Krauze-Baranowska, J. Renata Ochocka, Barbara Król-Kogus
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Pharmaceutical Science
Secondary Metabolism
Uterine Cervical Neoplasms
030226 pharmacology & pharmacy
01 natural sciences
HeLa
0302 clinical medicine
Drug Discovery
Cytotoxic T cell
HaCaT Cells
Chromatography
High Pressure Liquid
Membrane Potential
Mitochondrial
Ovarian Neoplasms
steroidal saponins
biology
Chemistry
Drug Synergism
General Medicine
rtca
trigonella foenum-graecum
Biochemistry
Seeds
Molecular Medicine
Female
Research Article
musculoskeletal diseases
Trigonella
Fraction (chemistry)
RM1-950
03 medical and health sciences
Inhibitory Concentration 50
Cell Line
Tumor
Humans
Pharmacology
Flavonoids
Plant Extracts
flavone c-glycosides
Fabaceae
Saponins
biology.organism_classification
Antineoplastic Agents
Phytogenic
0104 chemical sciences
Leukemia
Lymphoid
010404 medicinal & biomolecular chemistry
Complementary and alternative medicine
Cell culture
Cancer cell
cancer cells
Therapeutics. Pharmacology
Reactive Oxygen Species
HeLa Cells
Zdroj: Pharmaceutical Biology, Vol 59, Iss 1, Pp 424-437 (2021)
Pharmaceutical Biology
article-version (VoR) Version of Record
ISSN: 1744-5116
1388-0209
Popis: Context Trigonella foenum-graecum L. (Fabaceae) has many therapeutic properties and anticancer potential. Objective The cytotoxic activities of standardized extracts and a fraction from fenugreek seeds and their compounds (sapogenins, flavone C-glycosides, alkaloid trigonelline) against human cancer SKOV-3, HeLa and MOLT-4 cells were evaluated. Materials and methods Fenugreek seeds were extracted with 70% methanol (A) or water (B). Furthermore, the seeds were purified with petroleum ether and chloroform and next extracted with methanol to obtain fraction (C). The quantitative analysis of saponins and flavonoids in the extracts was done with HPLC methods. The extracts (5–120 µg/mL) and compounds (1–50 µg/mL) were tested on the cells by MTT assay and RTCA system. The effect of a fraction on ROS production, mitochondrial membrane potential and caspase-3/7 activity in HeLa and SKOV-3 cells was also evaluated by flow cytometry. Results The strongest cytotoxic activity on cancer cells showed the fraction C (IC50 was 3.91 ± 0.03 for HeLa, 3.97 ± 0.07 for SKOV-3, and 7.75 ± 0.37 for MOLT-4) with the highest content of steroidal saponins (163.18 ± 11.03 μg/mg) and flavone C-glycosides (820.18 ± 0.05 μg/mg). The fraction significantly increased ROS production (up to four times higher than in keratinocytes as control) and caspases activity in the cells. The examined flavonoids did not exhibit the cytotoxic activity in contrast to yamogenin, tigogenin, and diosgenin. Conclusions The obtained results complement the data on the cytotoxic activity of Foenugraeci Semen and synergistic effect of flavonoids and saponins complex contained in the plant.
Databáze: OpenAIRE
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