Bradford quantification of Glomalin-Related Soil Protein in coloured extracts of forest soils
| Autor: | Siobhan Staunton, M. Nicolas, G. Cissé, M. Essi |
|---|---|
| Přispěvatelé: | Ecologie fonctionnelle et biogéochimie des sols et des agro-écosystèmes (UMR Eco&Sols), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université Félix Houphouët-Boigny (UFHB), Office national des forêts (ONF), Ministry of Higher Education and Research, Côte d’Ivoire., Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Office National des Forêts (ONF) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Soil Science
Humic substances 010501 environmental sciences [SDV.SA.SDS]Life Sciences [q-bio]/Agricultural sciences/Soil study 01 natural sciences [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture forestry [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry Organic matter Forest soils Bradford protein assay 0105 earth and related environmental sciences chemistry.chemical_classification Topsoil biology Coloured extracts Soil organic matter Extraction (chemistry) Quantification of GRSP 04 agricultural and veterinary sciences 15. Life on land Dilution Glomalin Autoclaved-citrate extractable protein chemistry Environmental chemistry Soil water 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries |
| Zdroj: | Geoderma Geoderma, 2020, 372, pp.114394. ⟨10.1016/j.geoderma.2020.114394⟩ Geoderma, Elsevier, 2020, 372, pp.114394. ⟨10.1016/j.geoderma.2020.114394⟩ |
| ISSN: | 0016-7061 1872-6259 |
| DOI: | 10.1016/j.geoderma.2020.114394⟩ |
| Popis: | Glomalin-related soil protein (GRSP) is thought to represent a fraction of recalcitrant organic matter in soil. But it is recognized that the autoclaved-citrate extraction procedure causes the co-extraction of humic substances which interfere (directly and indirectly) with the Bradford colorimetric assay. The aim of this work was to propose a reliable quantification method of GRSP from forest soil, very rich in organic matter and therefore in colour. We estimated the quantities of GRSP in the topsoil (0–10 cm) of 102 French forests using five methods: i) direct calibration, reasoned dilution with colour correction, ii) direct calibration, reasoned dilution but no colour correction, iii) direct calibration, 1:2 dilution, no colour correction, iv) indirect calibration and v) dilution method. Our results concur that the interference caused by the co-extracted compounds is not related simply to either the colour of the extracts or total soil organic matter content. These findings suggest that for improved accuracy of GRSP estimates using the Bradford method, extracts should be diluted, and the pH-specific absorbance of coloured extracts should be subtracted. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect