Fusion of the promoter region of rRNA operon rrnB to lac Z gene
| Autor: | Simi Kobi, Gad Glaser, Amos B. Oppenheim |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Operon
lac operon Guanosine Tetraphosphate medicine.disease_cause Escherichia coli Genetics medicine biology Structural gene Promoter DNA Restriction Enzymes Lambda phage Ribosomal RNA beta-Galactosidase biology.organism_classification Bacteriophage lambda Molecular biology Galactosidases Microscopy Electron Genes Biochemistry DNA Viral bacteria RRNA Operon |
| Zdroj: | Nucleic Acids Research. 8:4327-4336 |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | A Lambda phage was constructed in which the structural gene for beta galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon beta galactosidase synthesis in vitro is repressed by ppGpp. Repression of beta galactosidase synthesis by cAMP is reported. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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