Development of an enzyme-linked immunosorbent assay for the detection and measurement of the trypanocidal drug isometamidium chloride in cattle
| Autor: | F.J. Rowell, G.M. Urquhart, P.H. Holmes, A. Phillips, E.A. Gault, D.D. Whitelaw, I.A. Sutherland |
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| Rok vydání: | 1991 |
| Předmět: |
Male
chemistry.chemical_classification Drug General Veterinary Chemistry media_common.quotation_subject Enzyme-Linked Immunosorbent Assay Cross Reactions Pharmacology medicine.disease_cause Sensitivity and Specificity Trypanocidal Agents Cross-reactivity Phenanthridines HOMIDIUM BROMIDE chemistry.chemical_compound Enzyme medicine Animals Diminazene aceturate Cattle Trypanocidal Drugs Isometamidium chloride media_common Field conditions |
| Zdroj: | Research in Veterinary Science. 50:185-189 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(91)90103-u |
| Popis: | An enzyme-linked immunosorbent assay ( elisa ) was developed to measure accurately levels of the trypanocidal drug isometamidium in the serum of treated cattle. The assay requires only 5 Id of test serum, is sensitive to a level of 0·5 pg ml−1 and is highly specific. Cross reactivity does not occur with the two other widely used trypanocidal drugs diminazene aceturate and homidium bromide. Serum drug levels are detectable for up to six months in cattle after a single dose of 1 mg kg−1 intramuscularly, the maximum period under field conditions for which effective prophylaxis can be maintained against tsetse challenge. Application of the assay will aid the rationalisation of treatment campaigns and assist in assessing the occurrence of drug-resistant trypanosome populations. |
| Databáze: | OpenAIRE |
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