Function of Golgi-centrosome proximity in RPE-1 cells
| Autor: | Barbara M. Waring, Christine Sütterlin, Kevin Wang, Kati Tormanen, Celine Ton |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Science
A Kinase Anchor Proteins Golgi Apparatus Retinal Pigment Epithelium Biology Autoantigens Cell Line 03 medical and health sciences symbols.namesake 0302 clinical medicine Ciliogenesis Organelle Humans Fragmentation (cell biology) Mitosis 030304 developmental biology Centrosome 0303 health sciences Multidisciplinary Membrane Proteins Cell migration Epithelial Cells Golgi apparatus Cell biology Transport protein Cytoskeletal Proteins symbols Medicine CRISPR-Cas Systems 030217 neurology & neurosurgery Gene Deletion |
| Zdroj: | PLoS ONE, Vol 14, Iss 4, p e0215215 (2019) |
| ISSN: | 1932-6203 |
| Popis: | The close physical proximity between the Golgi and the centrosome is a unique feature of mammalian cells that has baffled scientists for years. Several knockdown and overexpression studies have linked the spatial relationship between these two organelles to the control of directional protein transport, directional migration, ciliogenesis and mitotic entry. However, most of these conditions have not only separated these two organelles, but also caused extensive fragmentation of the Golgi, making it difficult to dissect the specific contribution of Golgi-centrosome proximity. In this study, we present our results with stable retinal pigment epithelial (RPE-1) cell lines in which GM130 was knocked out using a CRISPR/Cas9 approach. While Golgi and centrosome organization appeared mostly intact in cells lacking GM130, there was a clear separation of these organelles from each other. We show that GM130 may control Golgi-centrosome proximity by anchoring AKAP450 to the Golgi. We also provide evidence that the physical proximity between these two organelles is dispensable for protein transport, cell migration, and ciliogenesis. These results suggest that Golgi-centrosome proximity per se is not necessary for the normal function of RPE-1 cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|