The Group III Two-Component Histidine Kinase of Filamentous Fungi Is Involved in the Fungicidal Activity of the Bacterial Polyketide Ambruticin
| Autor: | Bruno Hamon, Nelly Bataillé-Simoneau, Anita Dongo, Thomas Guillemette, Philippe Simoneau, Leonard Katz, Béatrice Iacomi-Vasilescu, Claire Campion |
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| Přispěvatelé: | Unité de recherche Pathologie végétale et phytobactériologie, Institut National de la Recherche Agronomique (INRA), IFR 149 QUAlité et Santé du Végétal, Partenaires INRAE, University of Agricultural Sciences and Veterinary Medicine, Kosan Biosciences |
| Rok vydání: | 2009 |
| Předmět: |
EXPRESSION DE GENE
Antifungal Agents Histidine Kinase CHAMPIGNON PHYTOPATOGENE Mutant Mutation Missense Dioxoles Saccharomyces cerevisiae Mycology Fludioxonil Applied Microbiology and Biotechnology Neurospora crassa Microbiology HAMP domain AMBRUTICINE Pyrroles Protein kinase A Pyrans [SDV.EE]Life Sciences [q-bio]/Ecology environment Alternaria brassicicola Ecology biology GROUPE III HISTIDINE KINASE Genetic Complementation Test Histidine kinase Alternaria biology.organism_classification Mutagenesis Insertional Response regulator Amino Acid Substitution Biochemistry PROPRIETES ANTIFONGIQUES Protein Kinases Gene Deletion Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2009, 75 (1), pp.127-134. ⟨10.1128/AEM.00993-08⟩ |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00993-08 |
| Popis: | We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and to a lesser extent to the phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties and are thought to exert their toxicity by interfering with osmoregulation in filamentous fungi. Disruption of the osmosensor group III histidine kinase gene Ab NIK1 (for A. brassicola NIK1) resulted in high levels of resistance to ambruticin and fludioxonil, while a mutant isolate characterized by a single-amino-acid substitution in the HAMP domain of the kinase only exhibited moderate resistance. Moreover, the natural resistance of Saccharomyces cerevisiae to these antifungal molecules switched to sensitivity in strains expressing AbNIK1p. We also showed that exposure to fludioxonil and ambruticin resulted in abnormal phosphorylation of a Hog1-like mitogen-activated protein kinase (MAPK) in A. brassicicola . Parallel experiments carried out with wild-type and mutant isolates of Neurospora crassa revealed that, in this species, ambruticin susceptibility was dependent on the OS1-RRG1 branch of the phosphorelay pathway downstream of the OS2 MAPK cascade but independent of the yeast Skn7-like response regulator RRG2. These results show that the ability to synthesize a functional group III histidine kinase is a prerequisite for the expression of ambruticin and phenylpyrrole susceptibility in A. brassicicola and N. crassa and that, at least in the latter species, improper activation of the high-osmolarity glycerol-related pathway could explain their fungicidal properties. |
| Databáze: | OpenAIRE |
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