Dimethyl sulfoxide-free cryopreservation solution containing trehalose, dextran 40, and propylene glycol for therapy with human adipose tissue-derived mesenchymal stromal cells

Autor:	Yasutaka Fujita, Masuhiro Nishimura, Tamaki Wada, Natsuki Komori, Takeshige Otoi
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	Cryopreservation Propylene glycol Clinical Biochemistry Biomedical Engineering Mesenchymal stromal cells Trehalose Bioengineering Cell Biology Dextran Biotechnology
Zdroj:	Cytotechnology. 74(5):515-529
ISSN:	0920-9069
Popis:	We evaluated a dimethyl sulfoxide (Me2SO)-free cryopreservation solution to freeze human adipose-derived mesenchymal stromal cells (hADSCs). In the first experiment, we compared the combined effects of 3% trehalose (3 T) and 5% dextran (5D) in lactated Ringer’s solution (LR) as a cryopreservation base solution containing 10% propylene glycol (PG). The cell viability of hADSCs immediately after thawing was significantly higher (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fc073524903d5e9f74bccb1bea65c69 http://repo.lib.tokushima-u.ac.jp/117814 Zobrazit plný text záznamu Full text from SpringerLink