Paraoxonase-1 gene Q192R and L55M polymorphisms and risk of cardiovascular disease in Egyptian patients with type 2 diabetes mellitus
| Autor: | Eman Awadallah, Alshaymaa A. Ibrahim, Dalia Abd-El Haleem, Mona Kafoury, Dalia El-Lebedy, Ingy Ashmawy |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
biology business.industry Endocrinology Diabetes and Metabolism Paraoxonase Type 2 Diabetes Mellitus medicine.disease Cardiovascular disease PON1 Endocrinology Polymorphism (computer science) Internal medicine Diabetes mellitus Genotype Paraoxonase-1 gene Type 2 diabetes mellitus Internal Medicine medicine biology.protein Risk factor business Allele frequency Research Article |
| Zdroj: | Journal of Diabetes and Metabolic Disorders |
| ISSN: | 2251-6581 |
| Popis: | Background Increased oxidative stress or an impaired antioxidant defense mechanism may play a crucial role in the onset and progression of atherosclerosis. Recently, Paraoxonase −1 (PON1) which accounts for most of the antioxidant effect of high density lipoprotein (HDL) cholesterol has been presented as a potential therapeutic agent against atherosclerosis development. Allele frequencies for PON1 gene that influence enzyme concentration as well as activity differ greatly among ethnic groups and data from several studies showed ethnic variations in the interpretation of cardiovascular disease (CVD) associated with PON1 polymorphisms. In this work, we investigated PON1 Q192R and L55M polymorphisms in Egyptian patients with type 2 diabetes mellitus (T2DM) and its association with CVD. Methods The study included 184 subjects classified into 3 groups; T2DM, T2DM + CVD, and healthy controls. PON1 polymorphisms were genotyped by real-time PCR and PON1 concentration was assayed in serum by ELISA (enzyme linked immunesorbent assay). Results Genotype and allele frequencies of Q192R were significantly different between controls and diabetic patients. Frequency of QQ genotype was significantly higher in healthy controls, while QR and RR genotypes were significantly higher in diabetic patients (p = 0.02). Frequency of 55LL and LM genotypes were significantly higher in patients than in controls (p = 0.009). Q192R polymorphism associated with CVD in our diabetic patients (p = 0.01) and with low serum PON1 concentration (p = 0.04). Multiple logistic regression analysis revealed significant correlations between 192R and other independent CVD risk factors. Conclusion PON1 192R and 55 L alleles are associated with T2DM. Q192R polymorphism is associated with CVD and lower serum enzyme concentration and might represents a novel risk factor for CVD in Egyptian patients with T2DM. |
| Databáze: | OpenAIRE |
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