Interface of Phospholipid Polymer Grafting Layers to Analyze Functions of Immobilized Oligopeptides Involved in Cell Adhesion
| Autor: | Kazuhiko Ishihara, Kyoko Fukazawa, Misaki Ito, Yuuki Inoue |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
Polymers Phosphorylcholine Biomolecule 0206 medical engineering Biomedical Engineering Substrate (chemistry) Peptide 02 engineering and technology Polymer Adhesion 021001 nanoscience & nanotechnology Grafting 020601 biomedical engineering Biomaterials chemistry Cell Adhesion Biophysics 0210 nano-technology Cell adhesion Oligopeptides Phospholipids |
| Zdroj: | ACS Biomaterials Science & Engineering. 6:3984-3993 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.0c00518 |
| Popis: | The aim of this study was to design a material surface for use in the analysis of the behavior of biomolecules at the interface of direct cell contact. A superhydrophilic surface was prepared with poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), which was grafted onto a substrate with controlled polymer chain density. An arginine-glycine-aspartic acid (RGD) peptide was immobilized at the surface of the polymer graft surface (PMPC-RGD surface). Initial adhesion of the cells to this substrate was observed. The PMPC-RGD surface could enable cell adhesion only through RGD peptide-integrin interactions. The density and movability of the RGD peptide at the terminal of the graft PMPC chain and the orientation of the RGD peptide affected the density of adherent cells. Thus, the PMPC graft surface may be a good candidate for a new platform with the ability to immobilize biomolecules to a defined position and enable accurate analysis of their effects on cells. |
| Databáze: | OpenAIRE |
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