Ultra-short cyclized β-boomerang peptides: structures, interactions with lipopolysaccharide, antibiotic potentiator and wound healing
| Autor: | Sheetal Sinha, Vidhya Bharathi Dhanabal, Veronica Lavanya Manivannen, Floriana Cappiello, Suet-Mien Tan, Surajit Bhattacharjya |
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| Přispěvatelé: | School of Biological Sciences, Interdisciplinary Graduate School (IGS), Advanced Environmental Biotechnology Centre (AEBC), Nanyang Environment and Water Research Institute |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Inorganic Chemistry
Cyclic Peptide antibiotic potentiator MDR Gram-negative bacteria cyclic peptide lipopolysaccharide (LPS) Organic Chemistry Biological sciences [Science] General Medicine Antibiotic Potentiator Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications |
| Zdroj: | International Journal of Molecular Sciences; Volume 24; Issue 1; Pages: 263 |
| Popis: | Many antibiotics are ineffective in killing Gram-negative bacteria due to the permeability barrier of the outer-membrane LPS. Infections caused by multi-drug-resistant Gram-negative pathogens require new antibiotics, which are often difficult to develop. Antibiotic potentiators disrupt outer-membrane LPS and can assist the entry of large-scaffold antibiotics to the bacterial targets. In this work, we designed a backbone-cyclized ultra-short, six-amino-acid-long (WKRKRY) peptide, termed cWY6 from LPS binding motif of β-boomerang bactericidal peptides. The cWY6 peptide does not exhibit any antimicrobial activity; however, it is able to permeabilize the LPS outer membrane. Our results demonstrate the antibiotic potentiator activity in the designed cWY6 peptide for several conventional antibiotics (vancomycin, rifampicin, erythromycin, novobiocin and azithromycin). Remarkably, the short cWY6 peptide exhibits wound-healing activity in in vitro assays. NMR, computational docking and biophysical studies describe the atomic-resolution structure of the peptide in complex with LPS and mode of action in disrupting the outer membrane. The dual activities of cWY6 peptide hold high promise for further translation to therapeutics. Published version S.B. acknowledges funding support from Singapore-MIT Alliance for Research and Technology (SMART) ING-000834 BIO IGN, Singapore. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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