Evaluation of equine oocyte developmental competence using polarized light microscopy
| Autor: | Alessandro Ricci, Veronica Stabile, Alessia Bertero, Leila Vincenti, Alberto Revelli, Francesca Ritrovato, Francesca Evangelista, Riccardo Fortina, Tiziana Nervo |
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| Rok vydání: | 2017 |
| Předmět: |
polarized light microscopy
0301 basic medicine Embryology Fertilization in Vitro Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Oogenesis Equine oocyte polarized light microscopy In vitro maturation medicine Animals Horses Zona pellucida Equine oocyte Polarized light microscopy 030219 obstetrics & reproductive medicine Cumulus Cells Chemistry Obstetrics and Gynecology Cell Biology Oocyte In vitro maturation In Vitro Oocyte Maturation Techniques 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Oocytes Female Microscopy Polarization |
| Zdroj: | Reproduction (Cambridge, England). 153(6) |
| ISSN: | 1741-7899 |
| Popis: | The purpose of this study was to observe in vitro-matured equine oocytes with an objective computerized technique that involves the use of a polarized light microscope (PLM) in addition to the subjective morphological evaluation obtained using a classic light microscope (LM). Equine cumulus-oocyte complexes (COCs, n = 922) were subjected to different in vitro maturation times (24, 36 or 45 h), however, only 36-h matured oocytes were analyzed using CLM. The 36-h matured oocytes that reached maturity were parthenogenetically activated to evaluate the quality and meiotic competence. Average maturation percentages per session in groups 1, 2 and 3 (24-, 36- and 45-h matured oocytes respectively) were 29.31 ± 13.85, 47.01 ± 9.90 and 36.62 ± 5.28%, whereas the average percentages of immature oocytes per session were 28.78 ± 20.17, 7.83 ± 5.51 and 22.36 ± 8.39% respectively. The zona pellucida (ZP) birefringent properties were estimated and correlated with activation outcome. ZP thickness and retardance of the inner layer of the zona pellucida (IL-ZP) were significantly increased in immature oocytes compared with mature oocytes (P P P in vitro maturation (IVM) protocol allowed equine oocytes to reach maturity, and PLM observation of ZP can be used to distinguish mature and immature oocytes as well as activated and non-activated oocytes. |
| Databáze: | OpenAIRE |
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