Effects of etomidate in the adrenal and cytokine responses to hemorrhagic shock in rats

Autor: Luísa Mateus, Isabelle Goy-Thollot, Ronald S. Walton, Nuno M. Félix, Solange Gil, Ana Sofia Matos, Maria M. R. E. Niza
Přispěvatelé: UNIDEMI - Unidade de Investigação e Desenvolvimento em Engenharia Mecânica e Industrial, DEMI - Departamento de Engenharia Mecânica e Industrial
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: European Journal of Inflammation, Vol 14 (2016)
Popis: This work was supported by Fundacao para a Ciencia e a Tecnologia-FCT (Project PEst-OE/AGR/U10276/2014). Hemorrhagic shock (HS) induces a compensatory endocrine and cytokine response which aims to restore homeostasis. This response can be modulated by general anesthetics. To our knowledge, no studies have evaluated if etomidate modulates this response in experimental HS. After being premedicated with buprenorphine (0.05 mg/kg subcutaneously), male Wistar rats were anaesthetized with 5% isoflurane and divided into three groups: G1 (control, n = 16), G2 (n = 13), and G3 (n = 14). G2 and G3 were subjected to HS by collecting 30% of their blood volume and resuscitated 90 min later with the collected blood and normal saline, in a 1:3 ratio, respectively. G3 received etomidate (1 mg/kg IV) before HS. Blood gas analysis, adrenocorticotropic hormone (ACTH), corticosterone, and plasma levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-10 and of TNF-α, IL-6, and IL-10 mRNA obtained through real-time polymerase chain reaction (RT-PCR) were measured at 0, 90, 150, and 240 min after HS induction. Compared with G2, etomidate-treated animals had significantly lower corticosterone, PO2, PO2/FiO2, base excess and HCO3, and higher TNF-α, IL-6, IL-10, and TNF-α mRNA levels (P
Databáze: OpenAIRE