Skin collagen pentosidine and fluorescence in diabetes were predictors of retinopathy progression and creatininemia increase already 6years after punch-biopsy
| Autor: | P. Urios, Jacques Peyroux, M. Sternberg, Jocelyne M'bemba, Jean-Louis Selam, Anne-Marie Borsos, Gérard Slama |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Biopsy Clinical Biochemistry Urology 030209 endocrinology & metabolism Diabetic angiopathy Arginine Fluorescence Diabetic nephropathy 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Diabetes mellitus Internal medicine medicine Albuminuria Humans Diabetic Nephropathies Longitudinal Studies Pentosidine Skin Diabetic Retinopathy business.industry Lysine General Medicine Diabetic retinopathy Middle Aged medicine.disease 030104 developmental biology Endocrinology Diabetes Mellitus Type 1 Spectrometry Fluorescence chemistry Diabetes Mellitus Type 2 Case-Control Studies Creatinine Disease Progression Microalbuminuria Female Collagen medicine.symptom business Biomarkers Diabetic Angiopathies Retinopathy |
| Zdroj: | Clinical biochemistry. 49(3) |
| ISSN: | 1873-2933 |
| Popis: | Objective Advanced glycation end products (AGEs) of collagens appear to contribute to microvascular complications in diabetes. Do high concentrations of AGEs in skin collagen predict accelerated progression of these complications after 6 years and indicate the need for tighter anti-diabetic treatment? Design and methods We measured two AGE parameters in collagen extracted from skin punch-biopsies: pentosidine and fluorescence at 370/440 nm, as markers and predictors of microvascular complications, in 30 patients with diabetes (14 type-1, 16 type-2) without renal insufficiency, and in age- and gender-matched normoglycemic controls, followed at Hotel-Dieu in Paris. Results At the time of biopsy , marked increases in pentosidine (p = 0.0014) and fluorescence (p = 0.0001) expressed per collagen hydroxyproline, were found in the patients with diabetes versus the controls. A significant effect of age was found for pentosidine, but not fluorescence, measurements in the normoglycemic controls. Therefore pentosidine but not fluorescence results were corrected for age in the patients. Pentosidine and fluorescence were correlated with diabetes duration. Fluorescence was significantly dependent on retinopathy presence and score in type-1 and type-2 diabetes, whereas pentosidine was not. Fluorescence was correlated with microalbuminuria only in type-1 diabetes. Neither fluorescence nor pentosidine were correlated with creatininemia. Already six years after biopsy , retinopathy score progression and creatininemia increase were significantly correlated with initial pentosidine and fluorescence measurements. Conclusions These AGEs are good predictors of progression of microvascular complications and appear to be pathogenic. High skin concentrations of AGEs should induce tighter anti-diabetic treatment. |
| Databáze: | OpenAIRE |
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