A role for calcium-calmodulin-dependent protein kinase II in the consolidation of visual object recognition memory

Autor:	Malcolm W. Brown, JW Ho, Katherine E Narduzzo, Gareth R.I. Barker, E. C. Warburton, Chris J. Tinsley
Rok vydání:	2009
Předmět:	Male Time Factors Alpha (ethology) Cell Count Memory Ca2+/calmodulin-dependent protein kinase 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Perirhinal cortex medicine Animals Enzyme Inhibitors Phosphorylation Protein kinase A CAMK Infusion Pumps Recognition memory Neurons Analysis of Variance Kinase General Neuroscience Recognition Psychology Impaired memory Immunohistochemistry Rats medicine.anatomical_structure Exploratory Behavior Psychology Calcium-Calmodulin-Dependent Protein Kinase Type 2 Peptides Neuroscience
Zdroj:	The European journal of neuroscience. 30(6)
ISSN:	1460-9568
Popis:	The aim was to investigate the role of calcium-calmodulin-dependent protein kinase (CAMK)II in object recognition memory. The performance of rats in a preferential object recognition test was examined after local infusion of the CAMKII inhibitors KN-62 or autocamtide-2-related inhibitory peptide (AIP) into the perirhinal cortex. KN-62 or AIP infused after acquisition impaired memory tested at 24 h, indicating an involvement of CAMKII in the consolidation of recognition memory. Memory was impaired when KN-62 was infused at 20 min after acquisition or when AIP was infused at 20, 40, 60 or 100 min after acquisition. The time-course of CAMKII activation in rats was further examined by immunohistochemical staining for phospho-CAMKII(Thre286)alpha at 10, 40, 70 and 100 min following the viewing of novel and familiar images. At 70 min, processing novel images resulted in more phospho-CAMKII(Thre286)alpha-stained neurons in the perirhinal cortex than did the processing of familiar images, consistent with the viewing of novel images increasing the activity of CAMKII at this time. This difference was eliminated by prior infusion of AIP. These findings establish that CAMKII is active within the perirhinal region between approximately 20 and 100 min following learning and then returns to baseline. Thus, increased CAMKII activity is essential for the consolidation of long-term object recognition memory but continuation of that increased activity throughout the 24 h memory delay is not necessary for maintenance of the memory.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fbbe1ca33d0dba39996095ec68eae44 https://pubmed.ncbi.nlm.nih.gov/19735285 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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