Tissue Distribution of P-Glycoprotein in Cats
| Autor: | S. Van der Heyden, Koen Chiers, Richard Ducatelle |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Pathology medicine.medical_specialty medicine.drug_class Monoclonal antibody Epitope Epitopes Dogs Species Specificity Antibody Specificity medicine Animals Humans ATP Binding Cassette Transporter Subfamily B Member 1 P-glycoprotein CATS General Veterinary biology Adrenal cortex Antibodies Monoclonal General Medicine Molecular biology medicine.anatomical_structure Organ Specificity Cell culture Cats biology.protein Immunohistochemistry Female Antibody |
| Zdroj: | Anatomia, Histologia, Embryologia. 38:455-460 |
| ISSN: | 1439-0264 0340-2096 |
| DOI: | 10.1111/j.1439-0264.2009.00972.x |
| Popis: | Permeability glycoprotein (P-gp) is a membrane-bound efflux pump that exports various substances out of the cell. Variations in P-gp expression play an important role in susceptibility to toxic substances, drug efficacy and disease risk. In the present study, the distribution of the MDR1-gene product P-gp was determined in normal tissues of domestic shorthair cats using immunohistochemistry. Two monoclonal antibodies C494 and C219 were used, recognizing a different epitope on the human P-gp molecule. A consistent positive immunolabelling was obtained. The tissue distribution and cellular locations with antibody C494 were similar to those in man and dogs; with liver, colon, adrenal cortex and brain capillaries being consistently and intensely labelled. However, the immunolabelling in the kidney was in contradiction to man and dogs. The C219 antibody seems to react with a specific form of P-gp, only expressed in feline tissues with a barrier function, i.e. endothelia of the brain, testes and ovaries, and intestinal epithelial cells in contact with the lumen. |
| Databáze: | OpenAIRE |
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