Generation of leptin receptor bone marrow chimeras: recovery from irradiation, immune cellularity, cytokine expression, and metabolic parameters
| Autor: | Maria Pini, Melissa E. Gove, Christina L. Sherry, Giamila Fantuzzi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Leptin
Male medicine.medical_specialty Endocrinology Diabetes and Metabolism Interleukin-1beta Medicine (miscellaneous) Adipokine Adipose tissue Mice Obese Inflammation Thymus Gland Biology Chimerism Article Leukocyte Count Mice Endocrinology Immune system Bone Marrow Internal medicine medicine Leukocytes Animals Obesity Bone Marrow Transplantation Mice Knockout Nutrition and Dietetics Leptin receptor Body Weight Brain Transplantation Mice Inbred C57BL medicine.anatomical_structure Receptors Leptin Bone marrow Adiponectin medicine.symptom Spleen Signal Transduction |
| Zdroj: | Obesity (Silver Spring, Md.). 18(12) |
| ISSN: | 1930-739X |
| Popis: | Leptin regulates appetite and metabolism but also immunity and inflammation. Although functional leptin receptors (LepR) are expressed on hematopoietic cells, the role of these receptors in regulating immune function in vivo remains controversial. To clarify this issue, we performed bone marrow (BM) transplantation between obese db/db mice, lacking LepR, and wild-type (WT) mice. Results indicate that expression of LepR on BM-derived cells directly, though partially, regulates spleen and thymus cellularity, although the environment of db mice contributes to maintaining reduced cellularity of these organs. Selective expression of LepR on BM-derived cells also modulates leptin and adiponectin levels, with induction of a more favorable adipokine environment in the WT→db/db group. However, LepR signaling in BM-derived cells is not involved in regulation of body weight (BW) and composition, glycemia, hepatosteatosis or adipose tissue inflammation, although it modulates expression of interleukin (IL)-1β in the brain. Finally, data indicate that db mice have an increased susceptibility to irradiation compared to WT mice in terms of BW loss and recovery of leukocyte counts in peripheral blood. Therefore, interpretation of results obtained using BM chimeras between WT and db mice should take into account the difference in radiation sensitivity between the two types of animals. |
| Databáze: | OpenAIRE |
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