Distinct Responses of Splenic Dendritic Cell Subsets to infection with Listeria monocytogenes: maturation phenotype, level of infection, and T cell priming capacity ex vivo
| Autor: | Latoya M. Mitchell, Kristina L. Brzoza-Lewis, Jason M. Grayson, Curtis J. Henry, Elizabeth M. Hiltbold, Marlena M. Westcott |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
medicine.medical_treatment T cell Immunology Priming (immunology) Mice Transgenic Biology CD8-Positive T-Lymphocytes Lymphocyte Activation Article Flow cytometry Mice medicine Animals Listeriosis Mice Knockout Innate immune system medicine.diagnostic_test Dendritic cell Dendritic Cells Flow Cytometry Molecular biology Listeria monocytogenes Bacterial Load Mice Inbred C57BL Cytokine medicine.anatomical_structure Ex vivo CD8 Spleen |
| Popis: | To determine the relative contributions of DC subsets in the development of protective immunity to Listeria monocytogenes we examined the relationship between maturation, bacterial burden, and T cell priming capacity of four well characterized subsets of splenic DC following infection with Lm. CD8α(+), CD4(+), and CD8α(-)CD4(-) DC and the B220(+) plasmacytoid DC (pDC) were compared for abundance and costimulatory molecule expression at 24, 48, and 72h post i.v. infection. We further determined the bacterial burden associated with each DC subset and their relative capacities to prime CD8(+) T cells at 24hpi. The CD8α(+) DC displayed the highest level of maturation, association with live bacteria, and T cell activation potential. Second, the CD4(+) DC were also mature, yet were associated with fewer bacteria, and stimulated T cell proliferation, but not IFN-γ production. The CD8α(-)CD4(-) DC showed a modest maturation response and were associated with a high number of bacteria, but failed to induce T cell proliferation ex vivo. pDC displayed a strong maturation response, but were not associated with detectable bacteria and also failed to stimulate T cell activation. Finally, we measured the cytokine responses in these subsets and determined that IL-12 was produced predominantly by the CD8(+) DC, correlating with the ability of this subset DC to induce IFN-γ production in T cells. We conclude that Listeria-specific CD8(+) T cell activation in the spleen is most effectively achieved by infection-induced maturation of the CD8α(+) DC subset. |
| Databáze: | OpenAIRE |
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