Calcium mediated DNA binding in non-lamellar structures formed by DOPG/glycerol monooleate
| Autor: | José Teixeira, Daniela Uhríková, Juan Carlos Martínez, Lukáš Hubčík, Nina Kanjakova, Mária Klacsová, Alexander Búcsi, Sophie Combet |
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| Přispěvatelé: | Combet, Sophie, Comenius University in Bratislava, LLB - Matière molle et biophysique (MMB), Laboratoire Léon Brillouin (LLB - UMR 12), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ALBA Synchrotron light source [Barcelone], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2021 |
| Předmět: |
glycerol monooleate
Lipid Bilayers 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Glycerides X-Ray Diffraction Phase (matter) Scattering Small Angle Zeta potential Lamellar structure Surface charge Lipid bilayer Molecular Biology Small-angle X-ray scattering Chemistry SANS Vesicle Organic Chemistry fungi Hexagonal phase Water Phosphatidylglycerols Cell Biology SAXS DNA 021001 nanoscience & nanotechnology 0104 chemical sciences [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry Crystallography [CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry DOPG Calcium 0210 nano-technology [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft] |
| Zdroj: | Chemistry and Physics of Lipids Chemistry and Physics of Lipids, 2021, 239, pp.105118 Chemistry and Physics of Lipids, Elsevier, 2021, 239, pp.105118 Chemistry and Physics of Lipids, Elsevier, 2021, 239, pp.105118. ⟨10.1016/j.chemphyslip.2021.105118⟩ Chemistry and Physics of Lipids, 2021, 239, pp.105118. ⟨10.1016/j.chemphyslip.2021.105118⟩ |
| ISSN: | 1873-2941 0009-3084 |
| DOI: | 10.1016/j.chemphyslip.2021.105118⟩ |
| Popis: | International audience; In order to test an encapsulation method of short fragmented DNA (~ 20-300 bp), we study the solubilisation in 150 mM solution of NaCl of a cubic phase formed by glycerol monooleate (GMO) with negatively charged dioleoylphosphatidylglycerol (DOPG) up to the level of unilamellar vesicles and, subsequently, the restoration of the cubic phase using Ca 2+ cations. We performed small angle X-ray and neutron scattering (SAXS and SANS) to follow structural changes in DOPG/GMO mixtures induced by increasing DOPG content. The cubic phase (Pn3m space group) is preserved up to ~ 11 mol% of DOPG in DOPG/GMO. Above 20 mol%, the SANS curves are typical of unilamellar vesicles. The thickness of the DOPG/GMO lipid bilayer (d L) decreases slightly with increasing fraction of DOPG. The addition of 15 mM of CaCl 2 solution shields the electrostatic repulsions of DOPG molecules, increases slightly d L and restores the cubic structures in the mixtures up to ~ 37 mol% of DOPG. Zeta potential shows negative surface charge. The analysis of the data provides the radius of the water nano-channels of the formed non-lamellar structures. We discuss their dimensions with respect to DNA binding. In addition, Ca 2+ mediates DNA-DOPG/GMO binding. The formed hexagonal phase, H II , binds less of DNA in comparison with cubic phases (~ 6 wt% and ~ 20 wt% of the total amount, respectively). The studied system can be utilized as anionic Q II delivery vector for genetic material. |
| Databáze: | OpenAIRE |
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