Trefoil Factor-1 (TFF1) Enhances Oncogenicity of Mammary Carcinoma Cells
| Autor: | Jo K. Perry, Xiangjun Kong, Yulu Yang, Nagarajan Kannan, Juan Lin, Nethaji Muniraj, Prudence M. Grandison, Peter E. Lobie, Naeem Amiry, Dong-Xu Liu, Tao Zhu, Cécile M. Vouyovitch, Sahra Borges, Hichem C. Mertani |
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| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Cell Survival Mice Nude Breast Neoplasms Biology Cell morphology Mice Endocrinology Cell Movement Cell Line Tumor Internal medicine medicine Carcinoma Animals Humans Neoplasm Invasiveness RNA Messenger Viability assay Autocrine signalling Cell Proliferation Mice Inbred BALB C Cell growth Tumor Suppressor Proteins Cell Cycle Cell migration Cell cycle medicine.disease Cell culture Disease Progression Female RNA Interference Trefoil Factor-1 |
| Zdroj: | Endocrinology. 150:4473-4483 |
| ISSN: | 1945-7170 0013-7227 |
| Popis: | The functional role of autocrine trefoil factor-1 (TFF1) in mammary carcinoma has not been previously elucidated. Herein, we demonstrate that forced expression of TFF1 in mammary carcinoma cells resulted in increased total cell number as a consequence of increased cell proliferation and survival. Forced expression of TFF1 enhanced anchorage-independent growth and promoted scattered cell morphology with increased cell migration and invasion. Moreover, forced expression of TFF1 increased tumor size in xenograft models. Conversely, RNA interference-mediated depletion of TFF1 in mammary carcinoma cells significantly reduced anchorage-independent growth and migration. Furthermore, neutralization of secreted TFF1 protein by polyclonal antibody decreased mammary carcinoma cell viability in vitro and resulted in regression of mammary carcinoma xenografts. We have therefore demonstrated that TFF1 possesses oncogenic functions in mammary carcinoma cells. Functional antagonism of TFF1 can therefore be considered as a novel therapeutic strategy for mammary carcinoma. |
| Databáze: | OpenAIRE |
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