TRPV1 and TRPA1 Function and Modulation Are Target Tissue Dependent
| Autor: | Erica S. Schwartz, Derek C. Molliver, Brian M. Davis, Kathryn M. Albers, Pam Cornuet, Julie A. Christianson, Sacha A. Malin |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Cholera Toxin medicine.medical_specialty Time Factors Colon Wheat Germ Agglutinins Freund's Adjuvant TRPV1 Artemin TRPV Cation Channels Dermatitis Biology Article Mice Transient receptor potential channel chemistry.chemical_compound Transient Receptor Potential Channels Neurotrophic factors Internal medicine Neural Pathways medicine Glial cell line-derived neurotrophic factor Animals RNA Messenger Receptors Cytokine TRPA1 Cation Channel Fluorescent Dyes Peroxidase Skin Muscles General Neuroscience Long-term potentiation Colitis Mice Inbred C57BL Disease Models Animal Endocrinology Nerve growth factor Gene Expression Regulation nervous system chemistry Capsaicin biology.protein Cytokines Intercellular Signaling Peptides and Proteins Calcium psychological phenomena and processes |
| Zdroj: | Journal of Neuroscience. 31:10516-10528 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | The nerve growth factor (NGF) and glial cell line-derived neurotrophic factor (GDNF) families of growth factors regulate the sensitivity of sensory neurons. The ion channels transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential channel, subfamily A, member 1 (TRPA1), are necessary for development of inflammatory hypersensitivity and are functionally potentiated by growth factors. We have shown previously that inflamed skin exhibits rapid increases in artemin mRNA with slower, smaller increases in NGF mRNA. Here, using mice, we show that, in inflamed colon, mRNA for both growth factors increased with a pattern distinct from that seen in skin. Differences were also seen in the pattern of TRPV1 and TRPA1 mRNA expression in DRG innervating inflamed skin and colon. Growth factors potentiated capsaicin (a specific TRPV1 agonist) and mustard oil (a specific TRPA1 agonist) behavioral responses in vivo, raising the question as to how these growth factors affect individual afferents. Because individual tissues are innervated by afferents with unique properties, we investigated modulation of TRPV1 and TRPA1 in identified afferents projecting to muscle, skin, and colon. Muscle and colon afferents are twice as likely as skin afferents to express functional TRPV1 and TRPA1. TRPV1 and TRPA1 responses were potentiated by growth factors in all afferent types, but compared with skin afferents, muscle afferents were twice as likely to exhibit NGF-induced potentiation and one-half as likely to exhibit artemin-induced potentiation of TRPV1. Furthermore, skin afferents showed no GDNF-induced potentiation of TRPA1, but 43% of muscle and 38% of colon afferents exhibited GDNF-induced potentiation. These results show that interpretation of afferent homeostatic mechanisms must incorporate properties that are specific to the target tissue. |
| Databáze: | OpenAIRE |
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