Dual Aptamer-Functionalized in Situ Injectable Fibrin Hydrogel for Promotion of Angiogenesis via Codelivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor-BB
| Autor: | Ge Zhang, Xiaolong Zhang, Lidya Abune, James Coyne, Akiho Suzuki, Huizhen Jia, Na Xiong, Peng Shi, Yong Wang, Nan Zhao |
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| Rok vydání: | 2019 |
| Předmět: |
Vascular Endothelial Growth Factor A
Materials science Angiogenesis Aptamer medicine.medical_treatment Becaplermin Neovascularization Physiologic 02 engineering and technology 010402 general chemistry 01 natural sciences Article Fibrin Mice chemistry.chemical_compound In vivo Human Umbilical Vein Endothelial Cells medicine Animals Humans General Materials Science Mice Inbred BALB C biology Growth factor technology industry and agriculture Hydrogels Aptamers Nucleotide 021001 nanoscience & nanotechnology 0104 chemical sciences Vascular endothelial growth factor chemistry Drug delivery Self-healing hydrogels biology.protein Biophysics Female 0210 nano-technology |
| Zdroj: | ACS Appl Mater Interfaces |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.9b02462 |
| Popis: | In situ injectable hydrogels hold great potential for in vivo applications such as drug delivery and regenerative medicine. However, it is challenging to ensure stable sequestration and sustained release of loaded biomolecules in these hydrogels. As aptamers have high binding affinities and specificities against target biomolecules, we studied the capability of aptamers in functionalizing in situ injectable fibrin (Fn) hydrogels for in vivo delivery of two growth factors including vascular endothelial growth factor (VEGF) and platelet-derived growth factor-BB (PDGF-BB). The results show that aptamer-functionalized fibrinogen (Fg) could form in situ injectable Fn hydrogels with porous structures. The aptamer-functionalized Fn hydrogels could sequester more VEGF and PDGF-BB than the native Fn and release these growth factors in a sustained manner with high bioactivity. After the aptamer-functionalized Fn hydrogels were subcutaneously injected into mice, the co-delivery of VEGF and PDGF-BB could promote the growth of mature blood vessels. Therefore, this study has successfully demonstrated that aptamer-functionalized in situ injectable hydrogels hold great potential for in vivo co-delivery of multiple growth factors and promotion of angiogenesis. |
| Databáze: | OpenAIRE |
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