Metabolite identification in human liver needle biopsies by high-resolution magic angle spinning1H NMR spectroscopy
| Autor: | Juan A. del Olmo, Bernardo Celda, Paloma Lluch, Daniel Monleon, Luis Martí-Bonmatí, B. Martínez-Granados, Antonio Ferrández, José M. Rodrigo, M. Carmen Martínez-Bisbal |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Liver Cirrhosis Male Magnetic Resonance Spectroscopy Metabolite Nuclear Overhauser effect Sensitivity and Specificity chemistry.chemical_compound Nuclear magnetic resonance Magic angle spinning Humans Radiology Nuclear Medicine and imaging Spectroscopy Aged Hepatitis Chronic Chemistry Biopsy Needle Reproducibility of Results Resonance Nuclear magnetic resonance spectroscopy Middle Aged NMR spectra database Proton NMR Molecular Medicine Female Spin Labels lipids (amino acids peptides and proteins) Protons Biomarkers |
| Zdroj: | NMR in Biomedicine. 19:90-100 |
| ISSN: | 1099-1492 0952-3480 |
| DOI: | 10.1002/nbm.1005 |
| Popis: | High-resolution magic angle spinning (HR-MAS) 1 H NMR spectroscopy of intact human liver needle biopsies has not been previously reported. HR-MAS NMR spectra collected on 17 specimens with tissue amounts between � 0.5 and 12 mg showed very good spectral resolution and signal-to-noise ratios. One-dimensional 1 H spectra revealed many intense signals corresponding to cellular metabolites. In addition, some high molecular weight metabolites, such as glycogen and mobile fatty acids, could be observed in some spectra. Resonance assignments for 22 metabolites were obtained by combining the analysis of three different types of 1D 1 H spectral editing, such as T2 filtering or the nuclear Overhauser effect and 2D TOCSY and 13 C-HSQC spectra. Biochemical stability of the liver tissue during up to 16 h of magic angle spinning at 277 K was studied. Biochemical trends corresponding to the different pathologies were observed, involving free fragments of lipids among other metabolites. NMR signal intensity ratios can be useful for discrimination among non-pathological, hepatitis C affected and cirrhotic liver tissues. Overall, this work demonstrates the applicability of HR-MAS NMR spectroscopy to the biochemical characterization of needle biopsies of the human liver. Copyright # 2006 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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